FastQCFastQC Report
Mon 22 Apr 2019
000000000-BY6FC_l01_n02_ISO_525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY6FC_l01_n02_ISO_525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7971048
Sequences flagged as poor quality0
Sequence length151
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATAATAATAGTTTTTTAAGATACAGGATCTCACCCCAGGCTGAAGTGC224290.2813808171773649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG3753.092282E-1023.2145
CCACCAC223350.015.094023145
AAGCGGG3901.05045634E-414.871795145
TAATAGT363750.011.91890057
AATAGTT347050.011.6568228
ATAGTTT360650.011.2574529
ATAATAG531000.010.6497176
GGCAGGG27900.010.134409145
CGGAGGG6600.0085732088.787879145
CTGGGGG22551.8189894E-128.359201145
TAATAGG52550.08.2778317
GCCGTAT19654.3291948E-108.117048145
GTATCTT169250.06.9822755145
TAAGGAG51400.06.77042779
CCGTATC18705.7213765E-66.590909145
TATAAGG121250.06.33814437
AGATCTC55300.06.292948145
GGTCGCC35253.45608E-116.1702127145
CGCCGTA17650.06.1614733140-144
GAGAGGG18652.5843823E-45.8310997
Produced by FastQC (version 0.11.5)