FastQCFastQC Report
Mon 22 Apr 2019
000000000-BY6FC_l01_n02_ISO_349.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY6FC_l01_n02_ISO_349.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4214804
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATAACAGTGATATTATTATACAAACGTGTAATATATATATATA49190.11670768083165907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG2951.4308171E-824.5762441
ATCGGGA4358.5958527E-716.6666472
CTCGGGG4251.2707214E-515.352923145
TCTCGGG3355.604293E-415.149237145
TCGGGAA3650.001073053313.9040943
GAGAGGG11601.8189894E-1212.4999867
CTGTCCA36050.010.658795145
TACAGGG13804.292815E-109.9818735145
GGAGAGG16851.8189894E-129.89613156
GGGAGAG17855.638867E-108.5294035
GAGGGGC9452.479564E-48.4391449
AATAGCG40900.08.3312858
AAGGCGT1850.00186779077.8378286140-144
CGGGAGA10255.943016E-47.78047944
ATAGCGA45150.07.7076329
TAACAGT118450.07.58969169
ATAACAG120050.07.5489298
TGTCACT12558.146407E-57.509951145
AGACGGA13602.7200122E-57.4632275
TGGTCGC17702.4206183E-66.9632688145
Produced by FastQC (version 0.11.5)