FastQCFastQC Report
Mon 22 Apr 2019
000000000-BY6FC_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY6FC_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800259
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1001845.564977039414884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAT301.5999136E-596.66548
CGAACAT259.388471E-486.998851
CATTCGA259.388471E-486.998852
TTCGCAT259.388471E-486.998854
GCGCGAT353.4382512E-582.856051
AACGATC353.4382512E-582.856059
CCAACTA300.001935456272.50509145
GATCGTA300.001936096172.499051
GTCTAGC300.001936096172.499058
TAGTCGA406.66507E-572.499057
AGGTCCG300.001936096172.499051
GTCTTCG554.063244E-665.908211
GTCCTAG350.00356715462.1420363
ATTCGAG350.00356715462.1420363
GGCTCGA606.8083336E-660.4158741
CCATTCG606.8083336E-660.4158741
GAGCGAT502.01081E-457.999232
CGATCAC502.01081E-457.999235
TCTGATC400.00605198554.3742839
CTCGAAA400.00605198554.3742831
Produced by FastQC (version 0.11.5)