FastQCFastQC Report
Mon 22 Apr 2019
000000000-BY6FC_l01_n01_ISO_525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY6FC_l01_n01_ISO_525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7971048
Sequences flagged as poor quality0
Sequence length151
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1603982.012257359383609No Hit
TAATAATAATAGTTTTTTAAGATACAGGATCTCACCCCAGGCTGAAGTGC218980.2747192088167077No Hit
AAAAAAAAAAAATTAGGTGTGGTGGTGCATGCCTATAGTCCCAGCTACTT88480.11100171520733534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG2600.001813228216.7307681
CCACCAC224850.016.089615145
CGACGCT1151.0204203E-613.86956515-19
TAATAGT367750.011.4738277
AATAGTT347500.011.349648
ATAGTTT348550.011.2530489
ATAATAG528950.010.34833056
GGCAGGG30000.07.7333336145
TCCCTAC11602.6971515E-47.59
GTATCTT172050.07.163615145
CCGATGT37700.07.115384145
TAATAGG52600.07.02946767
TATCTCG30857.2759576E-126.815235145
TCACCGA45450.06.69967145
TAATAGC31101.8313585E-85.82797437
GCCTAAA52250.05.827751145
ACCGATG40600.05.714286145
CACCGAT42502.5465852E-115.6294117145
TAATAAT2776900.05.3678561
TATAAGG121050.05.33044247
Produced by FastQC (version 0.11.5)