FastQCFastQC Report
Mon 22 Apr 2019
000000000-BY6FC_l01_n01_ISO_349.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY6FC_l01_n01_ISO_349.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4214804
Sequences flagged as poor quality0
Sequence length151
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG774121.8366690360927818No Hit
TATATATATAACAGTGATATTATTATACAAACGTGTAATATATATATATA50240.11919889987766928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAC4301.4194853E-515.1744189
CGACGCT902.1822096E-514.49999915-19
TATAAGG10102.6739144E-1012.202977
TACCGTT4300.00367819111.8023258
AGAGCAC48950.010.5158328
TTCCCTA7452.1644695E-49.7315448
CGGAAGA54200.09.6309974
TCGGAAG54350.09.6044163
ATCGGAA55450.09.4138862
CTGTCCA37950.09.361001145
GATCGGA56600.09.2226151
AAGAGCA58250.09.21037
GAAGAGC58400.09.1866456
TACAGGG8751.075721E-49.114285145
TACACGA1450.00182543729.010-14
ACACTCT9703.3551172E-58.9690721
TCTTCCG1300.00756591148.92307820-24
TATAAGC8951.3756289E-48.9106147
TAGACTG6550.0080541738.8549614
ATAGACT8205.658625E-48.8414633
Produced by FastQC (version 0.11.5)