FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_vTC15B8B10B11_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_vTC15B8B10B11_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148969
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3610.24233229732360423No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3260.21883747625344874No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3230.21682363444743535No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2950.19802777759131093No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2280.1530519772570132No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT1890.12687203377883988No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1870.12552947257483102No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1870.12552947257483102No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1780.11948794715679102No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1770.11881666655478655No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1690.11344642173875101No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1680.11277514113674657No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1670.11210386053474213No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1600.10740489632071101No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1580.10606233511670214No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1500.10069209030066659No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG202.124596E-6145.01
TAGTAGA256.446684E-6116.02
GAGCGAA406.0917773E-790.6251
GCAAAAG3600.084.5833363
AGCGAAA1350.075.185191
CAAAAGC4250.073.3529364
AGCAAAA4600.067.771742
AGGCGTC451.1905378E-464.444446
TAGGCGT451.1905378E-464.444445
AGCAGGG2850.063.5964938
TATGAGT350.003560548262.1428576
AAGCAGG5400.059.0740747
CTAGACT502.0046697E-458.04
GTAGTAA502.0046697E-458.01
CTATGCT1153.8198777E-1156.7391329
AGGTAGT400.006040821354.3751
TAGAAGT400.006040821354.3754
TAAACTA400.006040821354.3755
GAGCAAA5600.054.3751
GGTAGTT400.006040821354.3752
Produced by FastQC (version 0.11.5)