FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_vTC15B8B10B11_rep1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_vTC15B8B10B11_rep1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128898
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3350.25989542118574377No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3110.24127604772766062No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2820.21877763813247683No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2320.1799872767614703No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT1960.1520582165743456No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1820.14119691539046378No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1620.12568077084206117No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1610.12490496361464104No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1550.12025012025012026No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1520.11792269856785986No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1500.1163710841130196No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1460.11326785520333908No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1460.11326785520333908No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT1360.10550978292913776No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1340.10395816847429751No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1300.10085493956461698No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1290.10007913233719685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG501.9281288E-10101.59
GAGCGAA554.129106E-1092.272721
GCAGGGT1600.086.093759
GCAAAAG3150.075.952383
AGCAGGG2300.072.499998
AAGCAGG4450.068.426977
TAAACTA754.809408E-967.6666645
TCGTACT754.809408E-967.6666642
CAAAAGC3950.066.075954
AGGCGTC350.003559457862.1428576
TAGGCGT350.003559457862.1428575
AGCAAAA3900.059.487182
AGCAGGC651.0882772E-555.769238
GGCGTCT400.006038976454.3757
ATATATT805.8334444E-754.3757
GCGTCTC400.006038976454.3758
TTTTTAA953.1108357E-853.4210551
GTATTAG553.2084718E-452.7272721
AATATAT858.8637535E-751.1764686
AGCGAAA1152.4556357E-950.434781
Produced by FastQC (version 0.11.5)