FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576809
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA16100.051.7864951
GCAAAAG19100.045.5504263
GCAGGTA11500.034.6744359
AGCAGGC7850.033.248918
AGCAGGG8450.032.6040428
CAAAAGC27300.031.8686124
GCAGGGT6050.029.959139
AGCAAAA28400.029.868412
AAGCAGG34750.029.6263477
AAAGCAG34850.028.9172216
AAAAGCA32100.028.0066535
GGATAGC6150.025.935355
GGACAGT7700.025.422466
GCAGGCA9750.025.2824339
GAGCGAA4400.024.7162841
GCAGGTC4400.024.7162849
TATGGAT7050.023.652842
CGAAAGC8550.022.8950823
ACTGATC10700.022.360158
TGGATAG7200.022.1531124
Produced by FastQC (version 0.11.5)