FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_Neg_control_rep2_SID92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_Neg_control_rep2_SID92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAACCTGAGCGTAAATCCTTGCTGATCGTTCTTCCCATCCACACGTCA12.941176470588235No Hit
GCTGTAAGGAGGGGCTACAGTTTAAGGGTATGTTGAGATTATAGCATTTA12.941176470588235No Hit
TGTGACAAAGACATAAGGGAACCACAACCTGAATCACGATTACAGGTAAC12.941176470588235No Hit
CCCGGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAG12.941176470588235No Hit
GCCTTGCTATCCTCCTCGACAGTGTCGTCTGTCTTTGCGGCTATTTCTTC12.941176470588235No Hit
GTCGTAGAAAAACATAACTATGCGAAAGGATCAAGATATCCAGGACATTA12.941176470588235No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA12.941176470588235No Hit
AGCTGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC12.941176470588235No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCTGGAATTGCTGCATGTA12.941176470588235No Hit
GATGGAGACTGATGGAGAACGCCAGAATGCCACTGAAATCAGAGCATACG12.941176470588235No Hit
TACACAAGTAAATTAATGAGAGAGGAAGGAAGTGGATGAAAGAAGACACA12.941176470588235No Hit
TCCTTGTAATGTGGGGTATTAATCAACCGCAAAAAAGTAAGGAAAAACAG12.941176470588235No Hit
ATCCTGCCTGCCTGCCTGTGTGTATGGACCTGCCGTAGCCAGTGGGTACG12.941176470588235No Hit
CAATTGAGGCGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCT12.941176470588235No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC12.941176470588235No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC12.941176470588235No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGACCCCAAAGATCAAAGGTCAC12.941176470588235No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA12.941176470588235No Hit
GGATATGTGCTTCCTGATTTGGACAACCAGATCAGTCTACTGAAAAAACC12.941176470588235No Hit
CATCAATATTATTTGCTCAAAACTATTCTCTGTTATCTTCAGTTTGTGTC12.941176470588235No Hit
TCATTATGTATTCTGTTGCTCTGCAGTCAGAAATCTCTGATTTGAAAACA12.941176470588235No Hit
CTCTAATACTCATGTACGCTGACTCGTCAAACCCAGAAACCCCAAAACTG12.941176470588235No Hit
AATGTATAGTTTGTCAAAATTGTCATTGTTTGGCATAGTCACGTTCAGCA12.941176470588235No Hit
TTAAGTACCAACACCAAGTCTCCTTGACCAATTAGCCTTATCTCCTTTCG12.941176470588235No Hit
GTGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCT12.941176470588235No Hit
TCCATTAGAAATATTAGAGTCTTCTGAAGATTAAAATTCACACCATAGTT12.941176470588235No Hit
GTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAAGCAGTAATGA12.941176470588235No Hit
ACCACTTCCTCCTCTGATTGGTCCTCCCTGATCTCAGCGATGCACTTTCA12.941176470588235No Hit
TAATGGGAACGGGGATCCAAATAACATGGACAAAGCAGTTAAACTGTATA12.941176470588235No Hit
ATGGCTCTCTATACGACCTTACTTATCATACTTCTATAAGCCCACTTTTC12.941176470588235No Hit
ATCGGGTTCCTTCCATCCAAAGAATGTTCTCATGCCTTAGATTGCGTCAT12.941176470588235No Hit
CAATTAGCACATTAGCCTTCTATCCTTTCGCAAGGTTGCTCAGTTCATTG12.941176470588235No Hit
ATATGATGAAATCAAATGCATGAGAACATTATTTGGATGGAAGGAAACCA12.941176470588235No Hit
CCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCAG12.941176470588235No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)