FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_Neg_control_rep1_SID90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_Neg_control_rep1_SID90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCGAACTGTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAA22.564102564102564No Hit
GGAAACCATAGCCTCCACCATACTGGATATCCCGACTGGTATCCATGGAG11.282051282051282No Hit
ACAGAATGGTTTTAGCCAGCACTACAGCTAAGGCTATAGAGCAAATGGCT11.282051282051282No Hit
TTATCAATTTGCACTCGGGCAGGGGACCACACTAGACAACAAATATTCAA11.282051282051282No Hit
GCAGTGAGACACCTCTGATGTGAAATAATTCCTTCTCATGCTTGCAATGT11.282051282051282No Hit
CTTAAAAGCACTCAAGCAGCCATCGACCAAATCAATGGGAAATTGAACAG11.282051282051282No Hit
ATATGCACTCACTTGGAAGTATAAGTCGGTATTCAACAGCTTGTATGCAT11.282051282051282No Hit
TTATCACATCTCTACATGACCTGTGCGTACAGGAAAAGATACATGGAAAT11.282051282051282No Hit
GCGTAGGACAAGGATGGGGGATGAGTCGAAGATAGAGGCTCAACGAACAC11.282051282051282No Hit
CTCTTCCGTGCAAGCCCTTTGCTATATTCGAGTTTCCGGCCGGTCACAAA11.282051282051282No Hit
CTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGG11.282051282051282No Hit
CTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGC11.282051282051282No Hit
CCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAAT11.282051282051282No Hit
TCAATAGACAAGTCATAATTGACAGCGATAGTCGGTCAGGTTACTCTGGT11.282051282051282No Hit
TAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCC11.282051282051282No Hit
CCCATTCGTTTCTGATAGGCCTGCAAATTTTCAAGAAGATCATTTTTCAG11.282051282051282No Hit
CTCCATATGTGATCTTGTTTACGTTTTGAAAGGGCTTGTCATTGGGAATG11.282051282051282No Hit
CTGGTAGAGACTGTGACTCTCCCTGATGCTTGAACATCCAGGCTGGTTTG11.282051282051282No Hit
TTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATC11.282051282051282No Hit
AGTCATAGGTCTGTCGGCCTTGTGTCAGCTTGTCTACTCGTGTTTGCTGC11.282051282051282No Hit
ATTCTAGCATTTACTTCTTTGGACATTTGAGACAGCTTGCCCTCAATGTA11.282051282051282No Hit
CTTGCTCGTCTTTTTTTCAAATGCATTTACCGTCGCTTTACATACGGACT11.282051282051282No Hit
GAGCAAAAGCAGGTAGATATTGAAAGACGAGTCTTCTACACACGGCAGAA11.282051282051282No Hit
GTACAAATTCCCATTCATTAGGTCGATGGAGGCTTGAGAGCTTTTAAGCC11.282051282051282No Hit
AATCAGGAAGCACATATCCAGTGCTGAACGTGACTATGCCAAACAATGAC11.282051282051282No Hit
ACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT11.282051282051282No Hit
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTATGG11.282051282051282No Hit
CATTATTGAGCTTTTCCCACTTCGTATTTTGAAGTAACCCCGAGGAGCAA11.282051282051282No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC11.282051282051282No Hit
AAATAGGGAGGACCTGAAAATCGAAACAAACCAATTTGCAGCAATAGCTG11.282051282051282No Hit
TTATATAGGTCCTCCATTTTTCTTAGGATCTTTCCCCGCACTGGGCTGTT11.282051282051282No Hit
AATCTCATATTACTCGCTTAACGGACGCCACTACACACTCTCCTTAACAC11.282051282051282No Hit
CCTAATGTTGCCTTTTTGGCAGGCCCACATGATGAACCCCAACAAAGCAA11.282051282051282No Hit
TTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAAT11.282051282051282No Hit
GATTGTGAATGCACCCAATCATGAAGGGATTCAAGCCGGAGTCGACAGGT11.282051282051282No Hit
GTACTGATGTGTCCTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCC11.282051282051282No Hit
CACATATACTATCTGGACAAGGCCAATAAAATTAAATCTGAGAAAACACA11.282051282051282No Hit
GAACCATAAACGCATGGGCGCTGGTGAATCGATGAGTGAAAGTATCCATA11.282051282051282No Hit
GTTTCTGATAGGCCTGCAAATTTTCAAGAAGATCATTTTTCAGACCAGCA11.282051282051282No Hit
GTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGGCCTATCAGAAA11.282051282051282No Hit
CTATTGGACCATCCTTAACCCTGGAGACGACATTGTATTAACTCATAATT11.282051282051282No Hit
ATCGGGTTGAGTTGCGGTGCTCCAGTTTCGGTGTTTGTTGTCCATCTTCC11.282051282051282No Hit
GTTGTATATAGACCCGCACCAGTCGACACCACGACTCCACTTGAAGGCGA11.282051282051282No Hit
GCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGATCAAGTGATCCTC11.282051282051282No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT11.282051282051282No Hit
GTTTTGGATCAACCGTCCCTCATAATCACTGAGTTTGAGTTCGGTGCACA11.282051282051282No Hit
CAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTACAAAC11.282051282051282No Hit
CTACAGCTAAGGCTATGGCGCAAATGGCTGGATCGAGTGAGCAAGCAGCA11.282051282051282No Hit
AATCAACAGAGGGCATCTGCGGGCCAAATCAGAATACAACCTACGTTCTA11.282051282051282No Hit
CCCTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCG11.282051282051282No Hit
ATATGATGCAATCAAATGCATGAGAACATTCTTTGGCTGGATGACACCCA11.282051282051282No Hit
ATCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT11.282051282051282No Hit
ATTTTAGTATATACTAACTAAATCTAGAGGAAGTAAATACTCAATTAGAA11.282051282051282No Hit
GCCGAGGCCAGGGCGGTTGCAAGTCCGTATGTCCAACAGGTGCAAGGGCT11.282051282051282No Hit
ATCTGGAACTGGATGTTGAGTGCTTCATCTCTGTATAAATGACTAACACT11.282051282051282No Hit
ATACTGGATATCCCGACTTGTCTTCTGTATGAACTGCTGGGGAAGAATTT11.282051282051282No Hit
AAATCATATAGCGGTATTCCCTCTCCTTCATGAAAAGGGTCCTTAATGAT11.282051282051282No Hit
TTTTCCAAGCCCTCATCCGAAAGTGCACCAGGGCATTGGTTAAGGTGAAC11.282051282051282No Hit
CATAACTGGAATCAATGCTAGAAAAAGGTGCAAATCCTGTAATTTGACAT11.282051282051282No Hit
GACCCTAAGAGACTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTATTG11.282051282051282No Hit
ATGGAGGAAGCAATGCTTGCAAAAGGGGACCTGGTAGCGGTTTTTTCAGT11.282051282051282No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT11.282051282051282No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT11.282051282051282No Hit
GAGCAAAAGCAGGAGTGAAAATGAATCCAAATCAAAAGATAATAACCTGT11.282051282051282No Hit
GGAGAAGCCAGCAAACTATAATCCCGAATATCGGGTCCAGACCCTGGGTA11.282051282051282No Hit
GCACATCCTAAATTCACTATTTTTGCTGACTTAGCTCTTAAACGACGAAA11.282051282051282No Hit
TCCCCTCACGACGGGAGCCATTCCTTGCTCTTAAATTCTTCGACATTTGC11.282051282051282No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT11.282051282051282No Hit
ATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGGATAT11.282051282051282No Hit
GTTGAATACGACCTTCCCAATGGAACTTTCCTCCACTCCTTTGGGGGAAT11.282051282051282No Hit
CGCTTGCACCATTTGTCTAGCCTGACTAGCAACCTACATGGCCTCTGCTG11.282051282051282No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT11.282051282051282No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC11.282051282051282No Hit
TCCATGGCCTCTGCTGCTTGCTCACTCGATCCAGCCATTTGCTCCATAGC11.282051282051282No Hit
TTCCATGACAGAAGATAATTTGGATTTATTCCCTTTTCGTGTGGTTTAAC11.282051282051282No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT11.282051282051282No Hit
GACAGAGACTGATAGTAAGTTCTCTGTTAGGCAAGATGTTGTGGCAATGA11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTGA50.0145.06
CTCCATA50.0145.01
ATGTGAT50.0145.07
CCCGAGG50.0145.0145
ATATGTG50.0145.05
TCCATAT50.0145.02
CCATATG50.0145.03
GTGATCT50.0145.09
CATATGT50.0145.04
TGTGATC50.0145.08
Produced by FastQC (version 0.11.5)