FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_KJ_G10_SID79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_KJ_G10_SID79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134166
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3630.2705603506104378No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2790.20795134385760924No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2540.18931771089545787No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2470.1841002936660555No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2410.17962822175513915No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2290.1706840779333065No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2170.16173993411147386No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2110.15726786220055752No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1840.13714353860143402No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1830.136398193282948No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1620.12074594159474084No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1550.11552852436533846No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1490.11105645245442212No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1430.1065843805435058No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1400.10434834458804765No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1360.10136696331410341No Hit
GAGCAAAAGCAGGAGTGAAAATGAATCCAAATCAAAAGATAATAACAATT1360.10136696331410341No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1350.10062161799561738No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG100.007084069145.04
AGGCGTC100.007084069145.06
GAGCGAA650.0100.384611
CTAGTAG608.221832E-1084.5833363
GCAGGGT1400.072.59
TAGTATA300.001932077872.51
AGCAGGG2000.068.8758
AAGCAGG4050.064.444447
GCAGGAG451.190108E-464.444449
GATAAGT350.00355977762.1428573
TAGTAGA851.2940291E-859.7058834
GAGCAAA3650.059.589041
CAAAAGC3300.059.318184
TACTGAT902.0316293E-856.388897
GGTCGTT400.006039516554.3757
AGTATAT400.006039516554.3752
ATATCAA400.006039516554.375145
ATAAGTT400.006039516554.3754
AGGTCGT400.006039516554.3756
GCAAAAG3700.052.9054033
Produced by FastQC (version 0.11.5)