FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_KJ_E10_SID78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_KJ_E10_SID78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120235
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT3790.3152160352642741No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2830.23537239572503846No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA2650.22040171331143177No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG2590.21541148584022954No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG2180.18131159812034764No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT1980.16467750654967356No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1750.14554830124339835No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1730.14388489208633093No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA1720.14305318750779725No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1640.1363995508795276No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG1620.13473614172246018No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA1590.13224102798685908No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC1500.12475568678005572No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA1470.1222605730444546No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1440.11976545930885349No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA1430.1189337547303198No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC1410.11727034557325237No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1400.11643864099471868No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA1400.11643864099471868No Hit
GGGCTAGACTGTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACG1370.11394352725911756No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT1360.11311182268058387No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA1260.10479477689524681No Hit
AAGTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTT1240.1031313677381794No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1230.1022996631596457No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGAG100.007083158145.01
AGCGAAA608.221832E-1084.5833361
GCAAAAG1600.081.56253
CAAGGTA451.2262735E-680.555569
AGCAAAA2350.055.5319182
CTGTCGA400.006037993454.3759
ACAAGGT805.8301157E-754.3758
TACTGAT553.2076018E-452.7272767
AAGCAGG2800.051.7857137
GTACTGA858.858715E-751.176476
AGCAGGG1850.050.945958
CAAAAGC2700.048.3333364
CCAGCTC803.714954E-545.31253
CAGCTCT803.714954E-545.31254
CTATGCT855.3133866E-542.647069
CGAAAGC1201.9541403E-742.2916683
CACTGTG1201.9541403E-742.2916687
GCAGGGT1201.9541403E-742.2916688
GCAGGGG1557.2941475E-1042.0967759
ATAGGTC700.001052931941.428575
Produced by FastQC (version 0.11.5)