FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_E1_rep4_SID88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_E1_rep4_SID88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198183
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTGC100.007086603145.07
GAGCGAA1000.079.751
AGCGGTA350.003562285362.142857145
AGCGAAA1650.061.5151521
CGAAAGC1950.055.7692263
GCGAAAG2000.054.3752
GAGCAAA4450.047.2471921
CAAAAGC4550.044.6153874
CGAAGGT1801.8189894E-1244.305557145
AACGCTC855.3259406E-542.64706145
GCAAAAG4750.041.2105263
CTAACCG907.460705E-540.277786
CAGTACG750.001480514538.6666649
TCTATAC750.001480514538.6666643
ATATAAG1604.5578417E-836.256
ACCAGTA800.002032683536.258
TATAATA800.002032683536.252
TAACCGA1051.8483706E-434.5238087
GGTGTTT850.002736707534.117658
AGCAAAA6000.033.8333322
Produced by FastQC (version 0.11.5)