FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_C1_rep3_SID87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_C1_rep3_SID87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128991
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAC300.001931907172.5145
CGACTCC300.001931907172.5145
CGAAAGC1250.069.6000063
AGCGAAA1250.069.6000061
TATAAGT451.18993354E-464.444442
GCGAAAG1450.060.02
GCAAAAG2750.058.03
GAGCAAA2800.056.9642831
CAAAAGC3400.049.0441174
GAGCGAA450.00962025348.3333321
ATAACAC752.5381365E-548.3333323
AGCAAAA3550.044.9295772
CGCTGCG657.3127414E-444.615387145
CTACAAG700.001053217841.428571
TACTAAG750.001478594438.6666645
GGCTAAC750.001478594438.6666641
AAGCAGG4750.038.1578947
GTATTTA800.002030055336.251
ATTACTA800.002030055336.253
TCTAAAC800.002030055336.253
Produced by FastQC (version 0.11.5)