FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_B1_rep2_SID86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_B1_rep2_SID86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192941
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTGC100.0070864577145.0145
GAGCGAA800.090.6251
CAGTACG607.997369E-872.59
CGAAAGC1350.064.444443
GCAAAAG4650.060.8064543
CAAAAGC4950.057.1212124
AGCGAAA1500.053.1666641
GAGCAAA5900.050.381361
TAGAGCG604.9350946E-448.3333329
GCGAAAG1800.048.3333322
AAGCAGG7600.045.7894747
AGCAAAA6300.044.880952
TATACTA700.001054519841.428575
GTATATA1252.7037277E-740.6000021
AAAGCAG8650.039.3930636
AAAAGCA7250.039.05
ATATCGT750.001480416938.6666641
TATATTC1051.8481891E-434.5238083
GCAGGTA1905.3787517E-934.3421069
CTATACT850.002736528234.117654
Produced by FastQC (version 0.11.5)