FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_A1_rep1_SID85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_HK_IAV_EG_A1_rep1_SID85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182430
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA850.085.294111
CGAAAGC1350.064.444443
GAGCAAA4950.064.444441
GGTATAA502.0058696E-458.01
CAAAAGC5450.055.871564
GCAAAAG5950.052.3949553
GCGAAAG1700.051.1764682
AGCAAAA6500.049.076922
AAGCAGG7500.047.3666657
GTATAAG951.9121999E-645.7894741
AGCGAAA1851.8189894E-1243.108111
AAAGCAG8900.042.3595546
AAAAGCA7500.041.566665
AGCAGGC2350.037.021288
TAAGGTC800.0020322636.255
TTACACT1051.847796E-434.523808145
TAACCGA1102.4283874E-432.9545444
TATAAGG1102.4283874E-432.9545442
GTCGTAC950.004717276430.5263181
CTATGCT1204.045178E-430.2083349
Produced by FastQC (version 0.11.5)