FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_HA_control_rep2_SID91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_HA_control_rep2_SID91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117359
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT3870.3297574110208846No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG1920.16360057601036138No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC1690.1440025903424535No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT1650.1405942450089043No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC1460.12440460467454562No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA1450.12355251834115834No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT1440.12270043200777103No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA1420.12099625934099643No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT1380.11758791400744723No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA1360.11588374134067264No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT1350.11503165500728534No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG1290.10991913700696154No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG1230.10480661900663776No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA1220.10395453267325046No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC1200.10225036000647586No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT1180.10054618733970125No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG400.006037638554.3755
GAGTATG1101.6534614E-952.7272761
GAGTATT1203.5743142E-948.3333327
TATATAA450.00961811148.333332145
TGAGTAT1203.5743142E-948.3333326
AGTATGA1203.5743142E-948.3333322
CGTTTCG450.00961811148.333332145
AGTATTC1307.2559487E-944.6153878
GACAGGC1557.2759576E-1042.096777
ATCTGGG1903.6379788E-1241.9736861
TCTGGGA1651.3442332E-939.5454562
GTATGAG1502.5662303E-838.6666643
TAAAGTA951.0233543E-438.1578944
GATAATA1157.0890183E-637.8260882
AATACCG1406.5436325E-736.2500045
GTATTCA1604.5309207E-836.259
TGTTGTG1604.5309207E-836.257
CCATGTT1854.116373E-935.270274
CCCATGT1854.116373E-935.270273
GTTGTGC1655.93991E-835.1515168