Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-BTF83_l01_n02_Exp3_HA_control_rep2_SID91.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 117359 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT | 387 | 0.3297574110208846 | No Hit |
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG | 192 | 0.16360057601036138 | No Hit |
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC | 169 | 0.1440025903424535 | No Hit |
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT | 165 | 0.1405942450089043 | No Hit |
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC | 146 | 0.12440460467454562 | No Hit |
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA | 145 | 0.12355251834115834 | No Hit |
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT | 144 | 0.12270043200777103 | No Hit |
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA | 142 | 0.12099625934099643 | No Hit |
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT | 138 | 0.11758791400744723 | No Hit |
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA | 136 | 0.11588374134067264 | No Hit |
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT | 135 | 0.11503165500728534 | No Hit |
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG | 129 | 0.10991913700696154 | No Hit |
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG | 123 | 0.10480661900663776 | No Hit |
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA | 122 | 0.10395453267325046 | No Hit |
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC | 120 | 0.10225036000647586 | No Hit |
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT | 118 | 0.10054618733970125 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACACGTG | 40 | 0.0060376385 | 54.375 | 5 |
GAGTATG | 110 | 1.6534614E-9 | 52.727276 | 1 |
GAGTATT | 120 | 3.5743142E-9 | 48.333332 | 7 |
TATATAA | 45 | 0.009618111 | 48.333332 | 145 |
TGAGTAT | 120 | 3.5743142E-9 | 48.333332 | 6 |
AGTATGA | 120 | 3.5743142E-9 | 48.333332 | 2 |
CGTTTCG | 45 | 0.009618111 | 48.333332 | 145 |
AGTATTC | 130 | 7.2559487E-9 | 44.615387 | 8 |
GACAGGC | 155 | 7.2759576E-10 | 42.09677 | 7 |
ATCTGGG | 190 | 3.6379788E-12 | 41.973686 | 1 |
TCTGGGA | 165 | 1.3442332E-9 | 39.545456 | 2 |
GTATGAG | 150 | 2.5662303E-8 | 38.666664 | 3 |
TAAAGTA | 95 | 1.0233543E-4 | 38.157894 | 4 |
GATAATA | 115 | 7.0890183E-6 | 37.826088 | 2 |
AATACCG | 140 | 6.5436325E-7 | 36.250004 | 5 |
GTATTCA | 160 | 4.5309207E-8 | 36.25 | 9 |
TGTTGTG | 160 | 4.5309207E-8 | 36.25 | 7 |
CCATGTT | 185 | 4.116373E-9 | 35.27027 | 4 |
CCCATGT | 185 | 4.116373E-9 | 35.27027 | 3 |
GTTGTGC | 165 | 5.93991E-8 | 35.151516 | 8 |