000000000-BTF83_l01_n02_Exp3_HA_control_rep1

Basic Statistics

Measure	Value
Filename	000000000-BTF83_l01_n02_Exp3_HA_control_rep1_SID89.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	118383
Sequences flagged as poor quality	0
Sequence length	151
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT	639	0.5397734472010339	No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT	319	0.26946436566061005	No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA	309	0.26101720686247176	No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA	295	0.24919118454507827	No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG	252	0.2128684017130838	No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG	240	0.2027318111553179	No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC	229	0.19343993647736585	No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG	222	0.18752692531866907	No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA	210	0.17739033476090316	No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA	210	0.17739033476090316	No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC	207	0.1748561871214617	No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT	202	0.17063260772239258	No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT	195	0.16471959656369578	No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC	191	0.1613407330444405	No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT	178	0.1503594266068608	No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA	172	0.14529113132797783	No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC	170	0.14360169956835017	No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC	169	0.14275698368853637	No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC	169	0.14275698368853637	No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT	155	0.1309309613711428	No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA	153	0.12924152961151517	No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA	153	0.12924152961151517	No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT	152	0.12839681373170134	No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT	152	0.12839681373170134	No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG	149	0.12586266609225988	No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT	148	0.12501795021244605	No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT	147	0.12417323433263222	No Hit
ATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTGCCCAT	144	0.12163908669319075	No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT	137	0.11572607553449399	No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA	135	0.11403664377486632	No Hit
GATGAGGACTGTCGCATATTCCACCTGTTGAGGAACTCTGAACCAGCTCA	128	0.10812363261616954	No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT	125	0.10558948497672807	No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC	123	0.10390005321710043	No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT	123	0.10390005321710043	No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG	123	0.10390005321710043	No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT	122	0.10305533733728661	No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA	121	0.10221062145747278	No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG	121	0.10221062145747278	No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC	120	0.10136590557765895	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTGGT	40	6.638963E-5	72.5	4
GACGACT	40	6.638963E-5	72.5	145
AGTACTG	45	1.1895303E-4	64.44444	2
GAAAGTG	35	0.0035587407	62.142857	145
GTACTGG	50	2.002978E-4	58.0	3
ACTGGTC	50	2.002978E-4	58.0	5
TCGGGCC	45	0.009618311	48.333332	4
CATGGTC	260	0.0	47.403843	9
CCGCGCC	175	1.8189894E-12	45.571426	9
TACATCA	275	0.0	44.818184	4
AGTAGAT	130	7.2559487E-9	44.615383	7
ATCATGG	295	0.0	44.237286	7
ATACATC	285	0.0	43.245613	3
ATAATAC	170	3.8198777E-11	42.64706	3
TATATAT	120	1.9537947E-7	42.291664	2
GTATATA	120	1.9537947E-7	42.291664	1
GATAATA	175	5.2750693E-11	41.42857	2
CTGGTCG	70	0.0010528659	41.42857	6
TGGTCGA	70	0.0010528659	41.42857	7
TAATACC	195	3.6379788E-12	40.897438	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content