FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_CW_B04_SID83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_CW_B04_SID83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204259
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG28071.374235651795025No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA26471.2959037300681977No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA15810.7740173015632114No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC14160.6932375072824208No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12820.627634522836203No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA12290.6016870737641915No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC10850.5311883442100471No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG10450.5116053637783402No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT9890.4841891911739507No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT9720.4758664244904753No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG9550.46754365780699997No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC9410.4606896146559025No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT9150.4479606773752931No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG9050.4430649322673665No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT9020.4415962087349884No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC7880.38578471450462404No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA7800.38186811841828266No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG7120.34857705168438113No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA6760.330952369295845No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA6620.3240983261447476No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA6570.32165045359078426No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT6560.3211608790799916No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC6530.31969215554761354No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA5980.2927655574540167No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC5970.29227598294322404No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA5860.2868906633245047No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT5780.2829740672381633No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC5710.2795470456626146No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC5590.2736721515331026No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCC5380.2633910868064565No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG5310.2599640652309078No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC5140.2516412985474324No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA4750.2325478926265183No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG4470.2188398063243235No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA4340.2124753376840188No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC4280.2095378906192628No Hit
ACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT4280.2095378906192628No Hit
CTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGTC4190.2051317200221288No Hit
CCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG4130.20219427295737274No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG4070.1992568258926167No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTG4060.19876725138182408No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA3980.19485065529548268No Hit
TATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCT3960.19387150627389738No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGCCC3890.19044448469834868No Hit
GTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGA3850.18848618665517797No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC3780.1850591650796293No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC3690.18065299448249528No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT3630.17771554741773923No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTG3610.1767363983961539No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG3550.1737989513313979No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3550.1737989513313979No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT3530.17281980230981253No Hit
CATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGA3350.16400746111554448No Hit
GTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGC3240.1586221414968251No Hit
CACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA3210.1571534179644471No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA3110.1522576728565204No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG3070.1502993748133497No Hit
GGACAAGAGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTG3040.1488306512809717No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2910.142466182640667No Hit
GGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCT2840.1390391610651183No Hit
AGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGG2830.13854958655432562No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG2810.1375704375327403No Hit
GAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGG2800.13708086302194764No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA2750.13463299046798427No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCT2720.13316426693560626No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTT2700.1321851179140209No Hit
GGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCAT2670.13071639438164293No Hit
TCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACG2670.13071639438164293No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2590.12679979829530155No Hit
GATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGC2540.1243519257413382No Hit
GGGATGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCATACTTACT2530.12386235123054554No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2530.12386235123054554No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG2530.12386235123054554No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT2520.12337277671975286No Hit
TCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGG2500.12239362769816751No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2490.12190405318737486No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC2460.12043532965499684No Hit
GAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTG2450.11994575514420416No Hit
GTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTAT2420.11847703161182617No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAG2370.1160291590578628No Hit
AGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTC2320.11358128650389945No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC2320.11358128650389945No Hit
ACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGATGTG2230.10917511590676542No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA2230.10917511590676542No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2230.10917511590676542No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT2220.10868554139597277No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG2220.10868554139597277No Hit
GTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTGCGACATTA2210.1081959668851801No Hit
CCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTC2110.10330022177725338No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC2100.1028106472664607No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA2080.10183149824487539No Hit
GACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCA2080.10183149824487539No Hit
GGCTAATGTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGA2050.10036277471249737No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1600.0117.81251
AGCAAAA6700.0101.7164152
GCAAAAG7200.094.652783
CAAAAGC7950.085.7232744
GAGCAAA8250.082.606061
AGCGAAA3150.078.253971
AGTAGAA2050.077.8048861
GCAGGTC7550.077.7814569
AAGCAGG11500.076.282617
AAAAGCA9400.072.499995
AGCAGGG2300.072.499998
AGCAGGT9600.070.9895868
AGAAACA5050.064.603963
GTAGAAA4900.063.6224481
TAGAAAC5050.063.1683162
CGAAAGC4150.061.1445773
GCAGGGT1800.060.4166689
AAAGCAG14700.059.676876
TAAACTA7700.059.3181845
CCATCTC502.0064414E-458.0145
Produced by FastQC (version 0.11.5)