FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_CW_B03_SID82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_CW_B03_SID82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205814
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA22681.1019658526630842No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19140.9299658915331318No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA11370.5524405531207789No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT9080.4411750415423635No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC8900.43242928080694215No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG8160.39647448667243246No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA7770.3775253384123529No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC7700.3741242092374668No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCC7020.3410846686814308No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC6920.3362259127173079No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT6820.3313671567531849No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA6730.32699427638547424No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT6280.30512987454692103No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT6180.30027111858279804No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG5950.2890959798653153No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT5760.27986434353348166No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG5750.2793784679370694No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA5370.2609151952734022No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC5320.25848581729134074No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA5260.25557056371286696No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG5120.24876830536309483No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC5060.24585305178462108No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA4580.2225310231568309No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT4440.2157287648070588No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG4320.20989825765011125No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4220.20503950168598833No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC4190.20358187489675142No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA4150.20163837251110225No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4010.19483611416133015No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT3770.18317509984743505No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC3680.1788022194797244No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG3620.17588696590125064No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC3610.17540109030483836No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC3400.16519770278018017No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3340.1622824492017064No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA3330.16179657360529412No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA3250.15790956883399576No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTG3100.15062143488781132No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3050.14819205690574985No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC2890.14041804736315314No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2880.13993217176674083No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC2770.1345875402062056No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA2690.13070053543490723No Hit
TATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCT2650.12875703304925806No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT2640.12827115745284579No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2630.12778528185643348No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2620.12729940626002118No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG2610.12681353066360887No Hit
GTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGC2580.125355903874372No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTG2570.12487002827795972No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT2570.12487002827795972No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGCCC2550.12389827708513514No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2550.12389827708513514No Hit
ACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT2550.12389827708513514No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2520.12244065029589823No Hit
CCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG2480.12049714791024906No Hit
GTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGA2370.11515251634971382No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT2350.11418076515688923No Hit
CTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGTC2300.11175138717482776No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG2290.11126551157841545No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2270.11029376038559087No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG2200.1068926312107048No Hit
CATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGA2200.1068926312107048No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2160.10494912882505562No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC2140.10397737763223104No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2100.10203387524658186No Hit
TCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACG2090.10154799965016957No Hit
CAGTGAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCC2080.10106212405375727No Hit
CACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA2060.10009037286093268No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAGC100.0070868004145.0145
GAGCGAA1250.0116.01
AGCAAAA7950.082.075472
TAAACTA4700.080.212775
GCAAAAG8250.078.212133
AGCGAAA3100.077.177421
CTATTCG5000.076.859
AGTAGAA2000.076.1251
TCTACTA300.00193355172.59
GCAGGGT2000.072.59
GCAGGTC6750.070.8888859
GAGCAAA9150.070.519131
TTTTTAA5600.068.616071
AAGCAGG11950.067.3430947
TTTCCTA554.050162E-665.909096145
TTTAAAC5850.064.444443
AGCAGGC902.8012437E-1064.444448
CAAAAGC10100.063.8861434
TTGTCGT350.003562480762.1428571
TTAAACT6450.059.5736434
Produced by FastQC (version 0.11.5)