FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp3_C2_B05_SID84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp3_C2_B05_SID84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132157
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1840.13922834204771597No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1710.12939155701173602No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1630.1233381508357484No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT1590.12031144774775457No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1540.11652806888776228No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1500.11350136579976845No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1440.10896131116777771No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1370.10366458076378852No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1350.10215122921979161No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1350.10215122921979161No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1340.10139455344779316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACTCG100.0070839506145.0145
GAGCGAA608.221832E-1084.583331
GCAAAAG2350.070.957453
GCAGGGT1500.062.8333329
CCAGCTC753.7361133E-758.03
TCGTACT502.0038377E-458.02
AGCTCTA753.7361133E-758.05
TCTATGC753.7361133E-758.08
AGCAAAA2850.055.9649122
GGCAGCC400.006039318654.375145
GTAGGTG400.006039318654.3759
TTACGGA400.006039318654.375145
AGCAGGG2300.053.586968
AGCGAAA1004.661706E-850.752
AAGCAGG4200.050.0595257
CTATGCT901.3147965E-648.3333329
TAAGTTG752.5384816E-548.3333325
CCAAGTA450.00962077648.3333329
AGCAGGC450.00962077648.3333328
CAGTATT604.929415E-448.333339
Produced by FastQC (version 0.11.5)