FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID47_NL_Cnt5_D4_rep2_SID74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID47_NL_Cnt5_D4_rep2_SID74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170745
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC13360.7824533661307799No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT11010.644821224633225No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA8070.4726346305894755No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA7280.4263668042988082No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6600.3865413335676008No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA5540.3244604527218952No Hit
TTTCAAATCTGTGCTTCAAAAGTGCATTTGGATCACCAAGTTCTACGATT5320.3115757416029752No Hit
AGTCAGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCA5250.30747606079240974No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA5150.30161937392017335No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA4470.261793903188966No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA4260.2494948607572696No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA4060.23778148701279686No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA3800.2225541011449823No Hit
TCGTAGAACTTGGTGATCCAAATGCACTTTTGAAGCACAGATTTGAAATA3670.214940408211075No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG3620.2120120647749568No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA3530.20674104658994405No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA3380.1979560162815895No Hit
GTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGA3270.1915136607221295No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA3230.18917098597323495No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG3190.1868283112243404No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT3170.18565697384989313No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC3160.18507130516266948No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3140.1838999677882222No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC3070.17980028697765674No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2920.17101525666930217No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC2880.16867258192040763No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT2880.16867258192040763No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2840.16632990717151308No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC2790.16340156373539488No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA2790.16340156373539488No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2730.15988755161205306No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG2710.1587162142376058No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2690.15754487686315852No Hit
GAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCACTTTT2650.15520220211426397No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA2620.15344519605259305No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2560.14993118392925123No Hit
ATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAA2550.1493455152420276No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG2530.1481741778675803No Hit
GTTCTACGATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAA2500.1464171718059094No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA2480.14524583443146213No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATTGGGCAAGG2470.14466016574423848No Hit
GTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC2430.14231749099534394No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2410.14114615362089666No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT2380.13938914755922574No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG2370.13880347887200212No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA2350.13763214149755482No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2340.1370464728103312No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2320.13587513543588392No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT2290.13411812937421302No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT2270.13294679199976572No Hit
ATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGA2260.1323611233125421No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA2240.13118978593809483No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG2210.1294327798764239No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA2140.12533309906585843No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC2120.12416176169141117No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2080.12181908694251663No Hit
GATTTGAAATATTAGCAAAGTCGGTATTCAACAGCTTGTATGCATCTCCA2050.12006208088084569No Hit
TCAATGAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCA2050.12006208088084569No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG2030.11889074350639843No Hit
CTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAG2030.11889074350639843No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1990.11654806875750388No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG1970.11537673138305661No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1930.11303405663416206No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1890.1106913818852675No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA1870.10952004451082022No Hit
GTCGGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAG1840.1077630384491493No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA1810.1060060323874784No Hit
CCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATTG1800.10542036370025476No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT1790.10483469501303114No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT1760.1030776889513602No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC1750.10249202026413659No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1740.10190635157691294No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA1730.10132068288968929No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGC100.0070857485145.0145
GTTACTA100.0070857485145.0145
GAGCGAA502.2573658E-887.01
CGAAAGC1800.080.555563
AGTAGAA2100.079.404761
AGCGAAA1650.079.090911
GCAAAAG4350.066.6666643
GCCGCGA451.1910344E-464.44445145
GAGCAAA4300.064.069761
CAAAAGC4700.061.702134
TAAACTA2150.060.6976745
CAGCTCT651.0897853E-555.7692344
GGTGTGT400.006042327354.3756
CCAGCTC953.1188392E-853.4210553
AGCAGGG1500.053.1666687
AAGCAGG6600.052.7272727
GCAGGTC1650.052.7272729
CTATTCG2500.052.29
CTATGCT701.6900152E-551.7857139
TTTTTAA2550.051.1764681
Produced by FastQC (version 0.11.5)