FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID47_NL_Cnt5_D4_rep1_SID57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID47_NL_Cnt5_D4_rep1_SID57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157764
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT12980.8227479019294643No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC11030.6991455591896757No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA8690.5508227479019295No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA7820.49567708729494686No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA6760.42848812149793364No Hit
AGTCAGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCA5530.3505235668466824No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA5230.3315078218097919No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG5210.3302401054739992No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA4460.28270074288177277No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA4150.26305113967698585No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA3960.2510078344869552No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA3800.24086610380061357No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA3560.22565350777110113No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA3460.21931492609213762No Hit
TTTCAAATCTGTGCTTCAAAAGTGCATTTGGATCACCAAGTTCTACGATT3340.21170862807738142No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2940.18635430136152734No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2800.17748028701097843No Hit
GTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGA2780.17621257067518573No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG2620.16607083998884412No Hit
TCGTAGAACTTGGTGATCCAAATGCACTTTTGAAGCACAGATTTGAAATA2590.16416926548515506No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2550.16163383281356963No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT2550.16163383281356963No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2540.16099997464567328No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2530.16036611647777693No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2530.16036611647777693No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2490.15783068380619153No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG2450.15529525113460613No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA2390.15149210212722802No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC2360.14959052762353897No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA2320.14705509495195354No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2300.14578737861616084No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT2280.14451966228036814No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA2250.1426180877766791No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA2230.14135037144088639No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA2180.13818108060140463No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2170.13754722243350828No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA2140.13564564792981923No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2130.13501178976192288No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC2090.13247635709033745No Hit
CTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAG2090.13247635709033745No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2080.1318424989224411No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2060.1305747825866484No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2050.12994092441875205No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC2040.1293070662508557No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT2040.1293070662508557No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA1980.1255039172434776No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG1910.12106691006820314No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1870.11853147739661773No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1800.11409447022134327No Hit
GTTCTACGATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAA1760.11155903754975786No Hit
GTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC1760.11155903754975786No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1730.10965746304606881No Hit
ATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAA1730.10965746304606881No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA1710.10838974671027611No Hit
GAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCACTTTT1710.10838974671027611No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1690.10712203037448341No Hit
TCAATGAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCA1680.10648817220658706No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC1660.10522045587079436No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATTGGGCAAGG1620.10268502319920896No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1610.1020511650313126No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACAG100.007085243145.0145
GAGCAAA4600.0105.597831
GAGCGAA850.0102.3529361
ATGTATG353.4282908E-582.857148
GCAAAAG5700.082.675443
CAAAAGC5900.079.872894
AAGCAGG7800.072.57
GCAGGTC1900.072.59
AGCGAAA1900.068.684211
GCAGGGT1650.065.909099
AAGGTCG1009.094947E-1265.259
AAAGCAG8750.064.628576
ACATGTA451.19075514E-464.444456
AGCAGGG1700.063.9705858
CGAAAGC2050.063.6585353
AAAAGCA7750.062.6774255
TAAACTA1650.061.5151525
GTAGAAA2450.059.1836741
TCCCGGG502.0050345E-458.0145
CTATTCG1950.055.769239
Produced by FastQC (version 0.11.5)