FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID46_NL_Cnt4_D4_rep2_SID73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID46_NL_Cnt4_D4_rep2_SID73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140364
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT4780.3405431592146134No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4010.28568578837878655No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3700.2636003533669602No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3440.24507708529252514No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3210.22869111738052492No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2940.20945541591861158No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2380.16955914621982843No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2160.15388561169530648No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2070.14747371120800204No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1830.13037530990852356No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1730.12325097603374084No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1660.11826394232139295No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1650.11755150893391468No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1520.10828987489669717No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1460.10401527457182752No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1410.10045310763443617No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCA100.0070844158145.0145
GAGCGAA451.2273504E-680.555561
GCTAGAG300.001932264872.55
AACACGC300.001932264872.5145
GCAGGGT1550.070.161299
GCAAAAG4300.062.3837243
TCTAGTA350.0035601262.1428572
AGCAGGG2150.060.6976788
GAGCAAA4450.060.2809031
AAGCAGG5100.059.705887
CAAAAGC4600.058.315224
TATGCTA400.006040096754.3752
GATTGTG400.006040096754.3751
AGGGTGT858.869483E-751.1764686
CGAGGGT450.00962201148.3333364
TAGTAGA450.00962201148.3333364
AGATGTT604.9304665E-448.3333325
GGTACTG2500.046.3999985
TACTGAT2550.045.4901967
GTACTGA2900.045.06
Produced by FastQC (version 0.11.5)