FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID45_NL_Cnt3_D4_rep1_SID55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID45_NL_Cnt3_D4_rep1_SID55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163467
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4660.28507282815491813No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3930.2404154967057571No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA3380.20676956205227967No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT3280.200652119388011No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC3210.19636990952302302No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3080.18841723405947378No Hit
CTCTAATATTGCTAAGAGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2810.1719001388659485No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2750.1682296732673873No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2640.1615004863366918No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2530.15477129940599632No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2530.15477129940599632No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2430.14865385674172768No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2400.1468186239424471No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2380.14559513540959337No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA2360.14437164687673965No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2240.1370307156796173No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2070.12663106315036063No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC2060.12601931888393375No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1940.1186783876868114No Hit
CCTCAGCAGTATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACA1900.11623141062110395No Hit
TCTCTGTAGAGTTTCAGAGACTCGAACTGTGTTATCATTCCATTCAAGTC1740.10644350235827416No Hit
GATACAAAGAGGGCCTGCCACTTTCTGCTTGGGTATGAGCATGGACCAGT1690.10338478102613984No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT1660.10154954822685924No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1650.10093780396043238No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1650.10093780396043238No Hit
CTACAGAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACT1650.10093780396043238No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG100.007085474145.02
CCAATCG203.8602095E-4108.75145
GAGCGAA553.6379788E-12105.4545441
TCGTACT900.080.555562
TATAGGC451.2282599E-680.555567
TAGGCAT451.2282599E-680.555569
ACCCCTA300.001932836472.5145
GCAAAAG4250.063.1176453
CACATCG350.003561169162.142857145
TAGAACA350.003561169162.1428574
GTCAAAC350.003561169162.142857145
AGCAGGC702.3223583E-762.1428578
TTATATA606.7802375E-660.4166684
CAAAAGC4450.058.6516884
AAGAGTG753.7416248E-758.0000045
AAGCAGG5650.056.460187
ATAGAAT400.006041868654.3758
GTTCATG553.2110285E-452.7272727
GAGCAAA5650.050.0442471
GCAGGGT1600.049.843759
Produced by FastQC (version 0.11.5)