FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID42_NL_Ind2_D4_rep2_SID69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID42_NL_Ind2_D4_rep2_SID69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86518
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG9901.144270556416006No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT9631.1130631776046602No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT8781.0148177257911648No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC7730.8934556970803763No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA6710.7755611549041818No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA5450.6299267204512355No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA5190.5998751704847546No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG3650.42187752837559817No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT3610.4172542129961395No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT3380.39067014956425256No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2460.2843338958367045No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2450.28317806699183984No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2190.2531265170253589No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2080.24041239973184772No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA1990.23000994012806583No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG1950.2253866247486072No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA1730.1999583901615849No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1690.19533507478212628No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT1640.189555930557803No Hit
CCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCCAG1640.189555930557803No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1610.18608844402320907No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1600.1849326151783444No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1580.1826209574886151No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1540.17799764210915647No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1520.17568598441942718No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1490.1722184978848332No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1490.1722184978848332No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1450.16759518250537458No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1440.16643935366050994No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1440.16643935366050994No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA1430.1652835248156453No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT1420.16412769597078064No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG1420.16412769597078064No Hit
GGAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGGCT1400.16181603828105134No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA1380.15950438059132205No Hit
CTTTTCTTACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATAC1350.15603689405672808No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1350.15603689405672808No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1340.15488106521186343No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1320.15256940752213413No Hit
TTTCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATA1320.15256940752213413No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1290.14910192098754016No Hit
GGATAAAGAAAGAAGAGTTCACTGAGATCATGAAGATCTGTTCCACCATT1270.14679026329781086No Hit
GGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTC1250.14447860560808157No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1250.14447860560808157No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1240.1433227767632169No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT1190.13754363253889362No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1190.13754363253889362No Hit
AAATCAATCCGTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCAT1180.13638780369402898No Hit
GGTTCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTT1170.13523197484916433No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1160.13407614600429968No Hit
GGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCA1140.13176448831457035No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA1130.13060865946970573No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA1120.1294528306248411No Hit
GCAAGGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1120.1294528306248411No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC1120.1294528306248411No Hit
GTAGTAAACAAAGTCGGTATTCAACAGCTTGTATGCATCTCCACAACTAG1120.1294528306248411No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG1110.12829700177997644No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1110.12829700177997644No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT1110.12829700177997644No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1080.12482951524538247No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1060.12251785755565317No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG1030.1190503710210592No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1010.1167387133313299No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1010.1167387133313299No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1000.11558288448646524No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT1000.11558288448646524No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT990.11442705564160059No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT970.11211539795187128No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA970.11211539795187128No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT950.10980374026214197No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT950.10980374026214197No Hit
GTACATGAAGCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTG950.10980374026214197No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT950.10980374026214197No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC940.10864791141727732No Hit
GTATAAGGGAAAGTTGTGCTTATAGCATTTTGTGCTGGCACTTTTAAGAA930.10749208257241268No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA930.10749208257241268No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC920.10633625372754801No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT910.10518042488268337No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA910.10518042488268337No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT890.10286876719295406No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA880.10171293834808942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA1000.0108.751
CAGCTCT203.8539985E-4108.749994
GAGCGAA700.0103.571431
GAGCAAA3150.098.9682541
AGCAGGC1450.095.000018
GCAAAAG3500.089.0714343
CTAAATA259.357578E-487.01
GCAGGCA1600.086.093749
CAAAAGC3700.080.3378454
AAGCAGG4650.077.956997
TTCGTAA300.001929748672.5145
CAAGGGT607.945164E-872.58
TAAATAG300.001929748672.52
AAAGCAG5250.069.0476156
CGAAAGC1100.065.909094
AGCGAAA1100.065.909092
AAAAGCA4600.063.043485
CTATGCT350.003555501662.1428579
AGCTCTA350.003555501662.1428575
GTAGAAA1800.060.4166642
Produced by FastQC (version 0.11.5)