FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID42_NL_Ind2_D4_rep1_SID52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID42_NL_Ind2_D4_rep1_SID52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121623
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG14871.2226305879644475No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT13031.071343413663534No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT12050.9907665490902214No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA10010.8230351167131217No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC9270.7621913618312326No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA6520.5360828132836717No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA6310.5188163423036761No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG4880.4012398970589444No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT4800.3946621938284699No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT4550.3741068712332371No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3260.26804140664183584No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2910.23926395500850992No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2850.23433067758565404No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2810.23104182597041678No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2740.2252863356437516No Hit
CCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCCAG2280.18746454206852323No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG2280.18746454206852323No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA2130.17513134851138354No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA2070.17019807108852766No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1980.16279815495424385No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1970.16197594205043456No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1960.16115372914662524No Hit
TTTCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATA1920.15786487753138798No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1890.15539823881996004No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1890.15539823881996004No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1870.15375381301234142No Hit
GGAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGGCT1850.15210938720472278No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1820.14964274849329484No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG1800.14799832268567623No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA1780.1463538968780576No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1760.14470947107043897No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA1740.14306504526282035No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1730.14224283235901103No Hit
CTTTTCTTACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATAC1730.14224283235901103No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1730.14224283235901103No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1710.1405984065513924No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1700.13977619364758312No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA1680.13813176783996448No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT1670.13730955493615515No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT1660.13648734203234586No Hit
GGTTCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTT1600.13155406460948998No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1600.13155406460948998No Hit
GGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCA1600.13155406460948998No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1600.13155406460948998No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1600.13155406460948998No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA1590.13073185170568066No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1560.12826521299425273No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1500.12333193557139685No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1490.12250972266758754No Hit
GGATAAAGAAAGAAGAGTTCACTGAGATCATGAAGATCTGTTCCACCATT1490.12250972266758754No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG1440.11839865814854099No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT1430.11757644524473168No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1430.11757644524473168No Hit
GGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTC1410.11593201943711304No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1400.11510980653330373No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC1400.11510980653330373No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1380.1134653807256851No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1370.1126431678218758No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1330.10935431620663855No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA1290.10606546459140129No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1290.10606546459140129No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1280.105243251687592No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1250.10277661297616406No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1240.10195440007235473No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT1220.1003099742647361No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1220.1003099742647361No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG151.2273707E-4145.02
GAGCAAA5250.0121.523811
GAGCGAA501.8189894E-12116.01
GCAAAAG6250.0103.2400053
CAAAAGC6250.0100.924
TCGTACT301.5937878E-596.6666642
AGCAGGC2600.094.8076868
AAGCAGG7350.090.74837
GTCATGA259.3667884E-487.01
ATTAGTA259.3667884E-487.01
TAGTAGA259.3667884E-487.03
GCAGGGT1300.083.653859
CCTGGTG353.425124E-582.85714145
AGCGAAA1500.082.166671
AAAAGCA7900.078.924055
AGTAGAA1800.076.527781
GCAGGCA3150.075.9523859
AAAGCAG8900.074.943826
AGCAGGG1650.074.6969768
AGATAAA406.639695E-572.5145
Produced by FastQC (version 0.11.5)