FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID41_NL_Ind1_D4_rep2_SID68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID41_NL_Ind1_D4_rep2_SID68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72320
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT10741.4850663716814159No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT8231.1379977876106193No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC8111.1214048672566372No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA6630.9167588495575221No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA5840.8075221238938054No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA5540.7660398230088495No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG4280.5918141592920354No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA3160.4369469026548673No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2740.3788716814159292No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2700.3733407079646018No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA2680.37057522123893805No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2380.3290929203539823No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2260.3125No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2250.31111725663716816No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA2210.3055862831858407No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2050.283462389380531No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1990.2751659292035398No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1990.2751659292035398No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG1910.26410398230088494No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1880.25995575221238937No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT1870.25857300884955753No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG1860.2571902654867257No Hit
CCATGGAACAAATGTACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTC1840.25442477876106195No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1830.25304203539823006No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA1820.2516592920353983No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA1820.2516592920353983No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT1800.2488938053097345No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1770.24474557522123894No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1760.24336283185840707No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT1760.24336283185840707No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1710.23644911504424776No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC1710.23644911504424776No Hit
GCAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGC1610.22262168141592922No Hit
CCATGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAA1610.22262168141592922No Hit
CAATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG1590.21985619469026546No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA1580.21847345132743365No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1580.21847345132743365No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG1560.2157079646017699No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1550.21432522123893805No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1530.21155973451327434No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1520.21017699115044247No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1510.2087942477876106No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1500.2074115044247788No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT1490.2060287610619469No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1480.20464601769911503No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1450.20049778761061948No Hit
GTTCATCTTCAAGTACTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGT1430.19773230088495575No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA1400.19358407079646017No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA1400.19358407079646017No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG1380.19081858407079647No Hit
GGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGAC1370.1894358407079646No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA1360.18805309734513273No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA1320.18252212389380532No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1310.18113938053097345No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1300.17975663716814158No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG1290.17837389380530974No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA1280.17699115044247787No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT1220.16869469026548672No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA1210.16731194690265488No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1210.16731194690265488No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1190.16454646017699115No Hit
GATCTTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG1190.16454646017699115No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG1180.16316371681415928No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT1180.16316371681415928No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG1170.16178097345132744No Hit
GTTGACAGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAG1170.16178097345132744No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC1170.16178097345132744No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA1160.16039823008849557No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1140.15763274336283187No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1110.1534845132743363No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1100.15210176991150443No Hit
CCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATTG1100.15210176991150443No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1070.14795353982300885No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1060.14657079646017698No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA1060.14657079646017698No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1050.14518805309734514No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA1030.14242256637168144No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC1030.14242256637168144No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC1030.14242256637168144No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG990.136891592920354No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG990.136891592920354No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG980.13550884955752213No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT980.13550884955752213No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT980.13550884955752213No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT970.1341261061946903No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCC970.1341261061946903No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG960.1327433628318584No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA950.13136061946902655No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG940.12997787610619468No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG920.12721238938053098No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG920.12721238938053098No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGT910.12582964601769914No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC910.12582964601769914No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG910.12582964601769914No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT910.12582964601769914No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC910.12582964601769914No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCA890.1230641592920354No Hit
TAAAAGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACTGGAGA890.1230641592920354No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTAACTAGAAGGATTTTC880.12168141592920353No Hit
TCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAA880.12168141592920353No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT870.1202986725663717No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT870.1202986725663717No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG850.11753318584070797No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC840.1161504424778761No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC840.1161504424778761No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC830.11476769911504425No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA820.11338495575221239No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG820.11338495575221239No Hit
GTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGA810.11200221238938055No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCT790.10923672566371682No Hit
AAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA790.10923672566371682No Hit
ATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAA780.10785398230088496No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC770.1064712389380531No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT770.1064712389380531No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG770.1064712389380531No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC750.1037057522123894No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA750.1037057522123894No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG750.1037057522123894No Hit
ATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAA740.10232300884955751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3850.0129.935071
GAGCGAA750.0125.666671
AGTAGAA900.0120.8333361
GCAGGCA1750.0107.714299
AGCAGGC1750.0107.714298
CAAAAGC5100.096.6666644
GCAAAAG5100.096.6666643
TTACCCT301.5893946E-596.666664145
AAGCAGG6250.095.1199957
GCAGGTC1350.091.2962959
AAAAGCA5450.089.128445
AAAGCAG7000.084.928576
AGCGAAA1300.083.653852
AGCAGGT4500.082.1666648
CGAAAGC1400.077.678574
ATTAGCT300.001928461872.5145
TAAACTA903.6379788E-1272.55
TTATAAG300.001928461872.53
GACCAGG300.001928461872.56
GCAGGTA3500.072.59
Produced by FastQC (version 0.11.5)