FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID37_Shed_Cnt2_D4_rep1_SID50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID37_Shed_Cnt2_D4_rep1_SID50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169171
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA6370.376542078725077No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG5090.30087899226226716No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT4000.23644714519628068No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3440.20334454486880138No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2990.17674424103421982No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2730.16137517659646156No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2440.14423275856973122No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2430.14364164070674051No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2420.1430505228437498No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2360.1395038156658056No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT2340.1383215799398242No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2260.1335926370358986No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT2210.1306370477209451No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2120.12531698695402876No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG2120.12531698695402876No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT2090.12354363336505665No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT2000.11822357259814034No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1990.11763245473514965No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1930.11408574755720544No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1910.11290351183122402No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1910.11290351183122402No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1870.11053904037926121No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC1850.10935680465327981No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1840.10876568679028913No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1840.10876568679028913No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1740.1028545081603821No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1720.10167227243440069No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1710.10108115457141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTG100.007085692145.0145
AGTTAGA100.007085692145.0145
CGAAGCG100.007085692145.0145
AGCAGGC256.449234E-6116.08
GAGCGAA301.5956013E-596.6666641
TAGTAAA451.2284454E-680.555562
AAGCAGG5800.061.257
GAGCAAA5350.060.981311
GCAAAAG5500.059.3181843
AGCGAAA1102.5465852E-1159.318181
CAAAAGC5600.058.258934
TTATATT502.0054512E-458.02
GCAGGGT1700.055.4411779
ATTATAT553.2113492E-452.7272721
GATAGTA553.2113492E-452.7272727
TAGTATA553.2113492E-452.7272729
AGCAGGT3750.052.1999978
TCGTACT701.6899494E-551.7857132
AGCAGGG2500.049.3000038
CGAAAGC1351.891749E-1048.3333363
Produced by FastQC (version 0.11.5)