FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID35_Shed_Ind2_D4_rep2_SID66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID35_Shed_Ind2_D4_rep2_SID66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125799
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG18721.4880881406052513No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT17051.3553366878910007No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA12651.0055723813384845No Hit
GGAACAGGATACACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGG11040.8775904418954046No Hit
GGAACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCT10360.8235359581554703No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT8610.6844251544129921No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC7070.5620076471196114No Hit
CTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTAT6890.5476991073060994No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA5980.47536148936001077No Hit
CAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTATCC5730.4554885173967997No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA5450.4332307887980032No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT5440.43243586991947475No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG4360.34658463103840254No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT4170.33148117234636204No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTATCCATGGTGTATCC3100.2464248523438183No Hit
CCATGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAAG2750.2186026915953227No Hit
CTTTTCTTACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATAC2460.19555004411799778No Hit
GATGGACAAATGTACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCC2450.1947551252394693No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA2290.1820364231830142No Hit
CCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCCAG2230.1772669099118435No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA2210.17567707215478662No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA2190.1740872343977297No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2180.17329231551920127No Hit
TCTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTA2170.17249739664067282No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2160.17170247776214437No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC2120.16852280224803057No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2100.1669329644909737No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG2090.16613804561244525No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2070.16454820785538837No Hit
GGTTCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTT2020.16057361346274612No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT2000.15898377570568925No Hit
GGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCA1950.155009181313047No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1860.147854911406291No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA1860.147854911406291No Hit
GCAAGGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1810.14388031701364876No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1760.13990572262100653No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1740.13831588486394963No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1700.13513620934983583No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1660.13195653383572206No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1630.1295717772001367No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1630.1295717772001367No Hit
CACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCC1630.1295717772001367No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG1620.12877685832160826No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1620.12877685832160826No Hit
GGATAAAGAAAGAAGAGTTCACTGAGATCATGAAGATCTGTTCCACCATT1610.12798193944307984No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1590.12639210168602294No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1580.1255971828074945No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACCAGTCGGGA1560.12400734505043759No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1530.12162258841485227No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG1530.12162258841485227No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1510.12003275065779538No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1490.11844291290073847No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1490.11844291290073847No Hit
GGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTC1380.10969880523692557No Hit
ATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGAC1380.10969880523692557No Hit
GAACAGGATACACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGC1380.10969880523692557No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1330.10572421084428334No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1320.1049292919657549No Hit
GGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAA1310.10413437308722645No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1280.1017496164516411No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT1270.10095469757311266No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1270.10095469757311266No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1270.10095469757311266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGC100.007083545145.0145
GCGCGGA100.007083545145.0145
TATATCG100.007083545145.05
CGGATTA100.007083545145.0145
GAGCGAA500.0145.01
CATTTCG151.2274696E-4145.0145
GGGCCCG203.8581158E-4108.75145
GAGCAAA4750.0103.789471
AGCAGGC3050.099.836078
GCAAAAG5100.096.6666643
AGTAGAA1550.093.5483861
CAAAAGC5600.088.035714
GTATAAA259.367537E-487.01
TCGTACT353.4255787E-582.857142
GTAGAAA1850.082.29732
AAGCAGG7050.080.212777
ACATGGT2200.075.795454
CATGGTT2300.072.55
TAGAAGT300.001931795772.54
TATAAAT300.001931795772.52
Produced by FastQC (version 0.11.5)