FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID35_Shed_Ind2_D4_rep1_SID49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID35_Shed_Ind2_D4_rep1_SID49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170984
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG24591.4381462592991157No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT23381.3673794039208347No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA16470.9632480232068497No Hit
GGAACAGGATACACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGG15720.9193842698732045No Hit
GGAACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCT12770.7468535067608665No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT11980.7006503532494268No Hit
CTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTAT10800.6316380480044916No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC9320.5450802414260983No Hit
CAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTATCC8980.5251953399148458No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT7100.41524353155850835No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA7080.4140738314696112No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA6940.4058859308473307No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG5960.3485706264913676No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT5520.3228372245356291No Hit
CCATGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAAG3820.22341271697936652No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTATCCATGGTGTATCC3730.21814906657932906No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT3560.2082066158237028No Hit
GATGGACAAATGTACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCC3530.206452065690357No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA3260.1906611144902447No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC3130.18305806391241286No Hit
CTTTTCTTACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATAC3100.18130351377906706No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA3000.17545501333458102No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2920.1707762129789922No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA2840.16609741262340336No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2840.16609741262340336No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2810.16434286249005756No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT2740.16024891217891732No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG2740.16024891217891732No Hit
TCTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTA2690.1573246619566743No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2670.1561549618677771No Hit
CCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCCAG2590.1514761615121883No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2550.14913676133439385No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA2540.14855191128994527No Hit
CACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCC2510.14679736115659944No Hit
GGTTCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTT2420.14153371075656201No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA2370.13860946053431902No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC2360.1380246104898704No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG2320.13568521031207598No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2290.13393066017873018No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2230.13042155991203855No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2190.12808215973424414No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG2190.12808215973424414No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2120.1239882094231039No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC2110.12340335937865532No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2080.1216488092453095No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2050.11989425911196369No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2040.1193094090675151No Hit
GGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCA2030.11872455902306649No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2000.11697000888972067No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG1980.11580030880082347No Hit
ATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGAC1970.11521545875637486No Hit
GCAAGGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1960.11463060871192626No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1920.11229120853413184No Hit
GTAGTAAACAGTATTTGCAACACTACAGGAGATATGCTTATAAGAAGTGC1910.11170635848968326No Hit
GAACAGGATACACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGC1870.10936695831188882No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1780.1041033079118514No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACCAGTCGGGA1780.1041033079118514No Hit
GGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAA1780.1041033079118514No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1740.10176390773405698No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1730.10117905768960839No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1720.10059420764515978No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGTC100.007085757145.0145
GAGCGAA800.0117.81251
AGCAGGC3900.0102.243588
GAGCAAA6150.096.6666641
GCAGGCA4450.089.606749
GCAAAAG6900.088.260873
ATCGGAG259.373345E-487.0145
CAAAAGC7800.079.00644
AAGCAGG9050.076.9060757
CGAAAGC1700.076.76473
CAATATA300.001932988672.5145
CAGTATC6100.067.7458951
CATGGTT2500.066.75
AGCGAAA1900.064.868421
CGGATTA451.1910398E-464.44444145
AGTATCC6700.062.7611922
ACATGGT2750.060.6363644
AAAGCAG12050.058.9626546
GCAGGGT1400.056.9642879
CTGTCTT902.0361767E-856.388885145
Produced by FastQC (version 0.11.5)