FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID34_Shed_Ind1_D4_rep2_SID65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID34_Shed_Ind1_D4_rep2_SID65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121705
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT22061.8125795982087836No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC16201.3310874655930323No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA12521.0287169795817757No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA11970.9835257384659627No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT11910.9785957848896922No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA11380.9350478616326362No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG7440.6113142434575408No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA6480.532434986237213No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA5190.4264409843473974No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC5110.4198677129123701No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA4940.40589951111293704No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT4470.3672815414321515No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA4400.3615299289265026No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC4310.35413499856209685No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA4180.34345343248017746No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA3810.3130520520931761No Hit
CAATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG3780.31058707530504087No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG3690.3031921449406351No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT3350.27525574134176906No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA3210.2637525163304712No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG3160.25964422168357915No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC3140.2580009038248223No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG3070.2522492913191734No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3050.2506059734604166No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC3050.2506059734604166No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA3050.2506059734604166No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA3040.2497843145310382No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT3040.2497843145310382No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG2900.23828108951974036No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA2870.2358161127316051No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2830.23252947701409143No Hit
GTTCATGCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGTA2830.23252947701409143No Hit
GCAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGC2800.2300645002259562No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA2800.2300645002259562No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT2790.2292428412965778No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG2790.2292428412965778No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2740.22513454664968574No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA2690.22102625200279363No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2670.2193829341440368No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2660.2185612752146584No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC2660.2185612752146584No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT2650.21773961628527996No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT2630.21609629842652317No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT2590.2128096627090095No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG2520.2070580502033606No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2470.20294975555646852No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA2470.20294975555646852No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA2450.20130643769771167No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2350.19308984840392754No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT2310.18980321268641387No Hit
GGAACTTTCCTCCACTCCTTTGGGGGACTCTCCAATGGGCCATGTTTCTG2310.18980321268641387No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG2310.18980321268641387No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA2310.18980321268641387No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA2300.18898155375703546No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG2240.18405160018076497No Hit
GGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGAC2170.17829998767511607No Hit
GCTTGTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATCC2160.17747832874573766No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG2100.17254837516946714No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT2080.17090505731071032No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG2070.1700833983813319No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT2030.16679676266381827No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG2030.16679676266381827No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA2020.16597510373443983No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC2000.164331785875683No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1990.1635101269463046No Hit
GTTCATTGATTCAATTTCTTTCTGTTTGGGCATAACCACTTGGTTCATTG1990.1635101269463046No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT1980.16268846801692619No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG1980.16268846801692619No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1980.16268846801692619No Hit
CCATGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAA1930.15858017337003408No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTAACTAGAAGGATTTTC1920.1577585144406557No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1920.1577585144406557No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA1900.15611519658189885No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG1860.1528285608643852No Hit
GGAAAGTTCCATTGGGAAGGTCTGCAGGACTTTATTAGCAAAGTCGGTAT1840.15118524300562838No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT1820.14954192514687153No Hit
GTTGACAGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAG1810.14872026621749312No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1800.14789860728811471No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1800.14789860728811471No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1780.14625528942935787No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC1750.14379031264122263No Hit
CAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTG1740.1429686537118442No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT1710.14050367692370896No Hit
GAAAAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTT1700.13968201799433055No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1690.13886035906495214No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC1620.13310874655930324No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1600.1314654287005464No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1580.12982211084178957No Hit
GTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGA1580.12982211084178957No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC1570.12900045191241116No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1550.12735713405365431No Hit
AAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA1550.12735713405365431No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA1550.12735713405365431No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1540.12653547512427593No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCT1530.1257138161948975No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC1520.1248921572655191No Hit
CCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATTG1520.1248921572655191No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC1510.12407049833614067No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT1500.12324883940676226No Hit
TCCTTCTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCATACT1500.12324883940676226No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT1490.12242718047738384No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCC1480.12160552154800543No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCA1470.120783862618627No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1450.11914054475987018No Hit
CCTTCTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCATACTC1430.11749722690111335No Hit
CATGTAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCG1430.11749722690111335No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1420.11667556797173492No Hit
CTCACTTGGAAGTATGCTTCTGAGAACAAATCAGAAACATGGCCCATTGG1420.11667556797173492No Hit
CCTCAATTGCTTCATATAGCCCCCCACAAGCTGTTGAATACCGACTTTGC1420.11667556797173492No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTC1410.11585390904235653No Hit
GGGCCATGTTTCTGATTTGTTCTCAAAGAACTCTTTGGTCATGTCTTTCT1400.1150322501129781No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC1370.11256727332484287No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC1360.11174561439546443No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT1340.11010229653670761No Hit
GTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC1330.1092806376073292No Hit
GATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTC1320.10845897867795078No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT1310.10763731974857238No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG1310.10763731974857238No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG1290.10599400188981553No Hit
GCTTCTTATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTG1290.10599400188981553No Hit
AGTTGTGGAGATGCATACAAGCTGTTGAATACCGACTTTGCTAATAAAGT1280.10517234296043712No Hit
CCATGTTTTTGGCTGGACCATGTGCTGGCATCATCACTGCATTGTTCATG1280.10517234296043712No Hit
AACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA1260.1035290251016803No Hit
CACTTGGAAGTATGCTTCTGAGAACAAATCAGAAACATGGCCCATTGGAG1240.10188570724292347No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA1230.10106404831354504No Hit
GGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCAT1220.10024238938416664No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC1220.10024238938416664No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG1220.10024238938416664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAA100.0070832656145.06
CGGGCTT100.0070832656145.0145
GAGCGAA950.0144.999981
GAGCAAA5550.0131.936941
AGCAGGC2350.0104.8936168
GCAAAAG7350.0100.612243
CAAAAGC7700.096.038964
GCAGGGT554.129106E-1092.2727369
AGCAGGG554.129106E-1092.2727368
GCAGGCA2650.090.283019
TAAACTA1850.090.135145
AAAAGCA8350.088.5628745
AAGCAGG9950.088.1658257
AAAGCAG10100.086.856436
AGTAGAA1600.081.56251
CTATTCG2250.077.333339
TTTTTAA2300.072.51
TTAGTAG300.001931645672.52
GACGCTC1000.072.5145
GACCTAC300.001931645672.5145
Produced by FastQC (version 0.11.5)