FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID33_Shed_Ind2_D3_rep2_SID64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID33_Shed_Ind2_D3_rep2_SID64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156127
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC3820.24467260627566023No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2660.170374118506088No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2520.16140705963734653No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2270.14539445451459387No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2140.13706789985076254No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2020.12938184939184127No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2010.12874134518693114No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1890.1210552947280099No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1830.11721226949854927No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1770.11336924426908863No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1680.10760470642489767No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1680.10760470642489767No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1670.10696420221998758No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1630.10440218540034715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG203.8598804E-4108.750012
TTACTAA259.3718094E-487.09
AGCGAAA1300.072.51
AGCAGGC955.456968E-1268.6842048
CGAAAGC1400.067.3214343
GGATCCG350.003560868662.14286145
TCGTACT606.7788133E-660.4166682
GCAGGGT1450.060.0000048
GAGCAAA3650.057.602741
GCAAAAG3700.054.864873
ATTAGTA400.00604136154.3750041
TATATTC400.00604136154.3750048
AATTATA400.00604136154.3750045
AATTACT400.00604136154.3750047
ATTATAT400.00604136154.3750046
CAAAAGC4250.049.470594
GCACTGT450.0096240248.3333368
ATATTCA450.0096240248.3333369
AGCAGGG2250.048.3333327
TAGGCAG951.9104136E-645.789475
Produced by FastQC (version 0.11.5)