FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID33_Shed_Ind2_D3_rep1_SID47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID33_Shed_Ind2_D3_rep1_SID47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167860
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4310.2567615870368164No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT3120.18586917669486475No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3110.1852734421541761No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2750.163826998689384No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2680.15965685690456333No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2610.15548671511974266No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2580.15369951149767663No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2540.15131657333492196No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG2500.1489336351721673No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2310.13761467889908258No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2300.1370189443583939No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2200.1310615989515072No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2160.12867866078875254No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2020.12033837721911116No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1990.11855117359704517No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1970.11735970451566782No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1870.11140235910878113No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1840.10961515548671512No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1820.10842368640533778No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1790.10663648278327177No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA1790.10663648278327177No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGCTCGCTGCCTTTTCGTC1690.10067913737638508No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGTA100.0070856432145.0145
GCAGGGT1400.0108.759
ATTATAT451.2284054E-680.555561
CGAAAGC1200.078.5416643
AGCAGGG2100.075.952388
ATTAGTA300.001932927272.51
TTATATT502.2978766E-672.52
AGCGAAA1350.069.814821
GCAAAAG4150.064.638553
GATGTAC451.1909767E-464.444446
TTCTATA350.003561335862.142857145
TTAGTAG350.003561335862.1428572
TCGTACT1007.1304385E-1058.02
GAGCAAA4750.057.9999961
CAAAAGC4800.055.8854184
TATATTC651.0897065E-555.7692343
CCTGAGT400.006042151254.375145
TGCCGCG400.006042151254.375145
GAGCGAA553.2112774E-452.7272761
AAGCAGG5850.052.051287
Produced by FastQC (version 0.11.5)