FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID31_Shed_Ind2_D2_rep2_SID62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID31_Shed_Ind2_D2_rep2_SID62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141257
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC3760.2661814989699625No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2950.20883920796845465No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2720.19255682904210059No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2590.1833537453011178No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2250.15928414167085525No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2250.15928414167085525No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2200.1557444940781696No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2060.1458334808186497No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1960.13875418563327835No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1810.12813524285522135No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT1780.12601145429960994No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1630.1153925115215529No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1570.1111449344103301No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1550.10972907537325585No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1520.10760528681764445No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1520.10760528681764445No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1520.10760528681764445No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1450.1026497801878845No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1450.1026497801878845No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1430.10123392115081022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA306.4574124E-10145.01
AGCAGGC1000.072.58
GCAAAAG3700.058.7837873
AAGCAGG4450.058.6516847
GAGCAAA3650.057.602741
CAAAAGC3900.055.769234
AGCGAAA1152.4574547E-950.4347841
GCAGGCA1600.049.843759
GTAGAAA1351.8735591E-1048.3333322
TAGTGTC450.00962213948.3333325
GATAGAT450.00962213948.3333323
GGATAGC951.9091112E-645.7894745
TATATAG1351.0153599E-842.962965
CGAAAGC1201.9573963E-742.2916683
CTAGTAG700.001053559741.428573
ATATAGG1451.907756E-840.0000046
TATGGAT1157.0992883E-637.8260882
TATAGGT1157.0992883E-637.8260887
ATGTATA1553.434252E-837.4193572
GGACAGT1553.434252E-837.4193576
Produced by FastQC (version 0.11.5)