FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID29_Shed_Ind2_D1_rep2_SID60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID29_Shed_Ind2_D1_rep2_SID60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100677
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1530.15197115527876276No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1490.14799805317997158No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1320.13111236926010905No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1120.11124685876615313No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1100.10926030771675754No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1100.10926030771675754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTT100.007081458145.08
GATAGTA259.362063E-487.07
TAGTATA259.362063E-487.09
TCGTACT502.2911827E-672.52
CCAGATA350.003557193662.1428574
ACAAGGT350.003557193662.1428573
TAAACTA400.00603514954.3755
TATGCTA400.00603514954.3752
CACTGCT400.00603514954.3751
CAAGGGT1205.820766E-1154.3754
GCTAGAG400.00603514954.3755
AGCAGGG805.8205296E-754.3758
AAGGGTG953.101559E-853.4210555
CTAGAGT553.205089E-452.7272766
GATATGT553.205089E-452.7272762
AGGGTGT1004.6467903E-850.756
GTCTAGT604.9237715E-448.3333321
ATATACT450.0096141648.3333324
AAGCAGG1800.048.3333327
GTCGTAC901.3116423E-648.3333321