FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID29_Shed_Ind2_D1_rep1_SID43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID29_Shed_Ind2_D1_rep1_SID43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114672
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2310.20144411887819172No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2140.1866192270126971No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2090.18225896469931632No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2020.17615459746058323No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1940.169178177759174No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1740.1517371285056509No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1580.1377842891028324No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1540.1342960792521278No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1540.1342960792521278No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1530.13342402678945164No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1510.13167992186409933No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1490.12993581693874703No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1310.11423887261057626No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1310.11423887261057626No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1220.10639040044649085No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1210.1055183479838147No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT1160.10115808567043394No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1150.10028603320775778No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGGT100.0070827343145.0145
GAGCGAA100.0070827343145.01
TTAGTAG100.0070827343145.02
GTAGTTT301.5933965E-596.6666648
GGTAGTT301.5933965E-596.6666647
AGGTAGT353.424286E-582.857146
TATAGGT607.968265E-872.54
TAGGTCC702.316192E-762.1428576
ATAGGTC851.2916644E-859.7058835
AGGGTGT851.2916644E-859.7058836
GGGTATA502.0027121E-458.01
CAAGGTA502.0027121E-458.04
TACTGAT502.0027121E-458.07
GTATATA753.7317113E-758.01
CAAAAGC2100.055.2380944
TATATAG805.827733E-754.3752
CTAACAT400.00603728454.3756
CAAGGCG400.00603728454.3759
TACTAAC400.00603728454.3754
ATATGTT553.2069755E-452.7272765
Produced by FastQC (version 0.11.5)