FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID28_Shed_Ind1_D1_rep2_SID59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID28_Shed_Ind1_D1_rep2_SID59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136232
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2260.16589347583533973No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2130.15635093076516532No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1840.13506371483939164No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1810.13286158905396675No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1770.12992542134006693No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1760.129191379411592No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1730.12698925362616711No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1570.11524458277056784No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1520.11157437312819309No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1500.11010628927124316No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1460.10717012155734337No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1430.1049679957719185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA406.64256E-572.51
GATGTAC451.19017306E-464.444446
TTAGTAT350.003559893762.1428572
GTAACAG502.0040566E-458.09
TTGATGT902.031993E-856.388894
AGGTAGT553.2091234E-452.7272726
GCAGGTC553.2091234E-452.7272728
CAAGGTA701.6881944E-551.7857134
AAGCAGG2950.049.1525427
CTGTACT1056.8475856E-848.3333324
GTACACC604.9299496E-448.3333329
CGAAAGC901.3150966E-648.3333323
CGACACT604.9299496E-448.3333324
GTACTGA1650.048.3333326
TCGACAC604.9299496E-448.3333323
GCTTTAG450.00962140648.3333321
TGTACTG951.9086056E-645.7894745
AGGGTGT1307.2705006E-944.6153836
TAAGGGT657.3141995E-444.6153834
AGGTCGC657.3141995E-444.6153837
Produced by FastQC (version 0.11.5)