FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID28_Shed_Ind1_D1_rep1_SID42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID28_Shed_Ind1_D1_rep1_SID42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180006
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3260.18110507427530192No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3180.17666077797406754No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT3140.17443862982345035No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3100.17221648167283313No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2970.1649945001833272No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2600.1444396297901181No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG2530.140550870526538No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2410.1338844260746864No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2290.1272179816228348No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2290.1272179816228348No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2260.1255513705098719No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2200.12221814828394609No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2140.11888492605802028No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT2090.11610724086974879No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT2040.11332955568147728No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT1890.10499650011666277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA203.8608516E-4108.750011
TTATATT353.4296092E-582.857142
ATTATAT353.4296092E-582.857141
TATATTC406.648364E-572.500013
CGAAAGC851.6916601E-1068.23533
TAGTGAT554.048101E-665.909097
TAAACTA451.19121076E-464.444445
CTACTAA350.003561755462.1428576
GGTAGTT400.006042858654.3750047
ACAAGGT953.1201125E-853.4210478
ATATTCA553.2119045E-452.7272724
CAAGGTA701.6903869E-551.7857139
GTAGAAA1900.049.605261
AGGTAGT450.00962640148.3333326
TACTGCC901.3174813E-648.3333327
TCCCGCA450.00962640148.333332145
GTAGTTT450.00962640148.3333328
CAAAAGC3650.047.6712344
AACGTAG803.722383E-545.3125049
AGCAGGG2400.045.31258
Produced by FastQC (version 0.11.5)