FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_SrcID27_SV_IAV_X31_D0_rep2_SID58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_SrcID27_SV_IAV_X31_D0_rep2_SID58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97771
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1660.1697844964253204No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1550.15853371654171483No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1270.12989536774708246No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1250.1278497714046087No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1220.12478137689089812No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1110.11353059700729255No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1060.1084166061511082No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1020.10432541346616071No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1010.10330261529492386No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC980.10023422078121323No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAA203.8555163E-4108.751
CAAGGTA651.544322E-978.076939
ACAAGGT754.794856E-967.666678
AGCAGGG1250.063.88
AGTGCAG350.003556887262.1428578
CGAAAGC502.001244E-458.03
AAGCAGG2750.057.9999967
CAAAAGC2650.057.452834
GTAGAAA1400.056.9642872
GCAGGGT902.0239895E-856.3888939
GCAAAAG2700.056.3888853
CACAGCG400.006034631354.3752
GTATAGT400.006034631354.3754
GTAGTGC400.006034631354.3756
AGTAGAA1500.053.1666681
TATAGGT553.204631E-452.7272767
TGTATAT1457.2759576E-1250.03
ATTAGCT450.00961333748.3333363
TTAGAGC450.00961333748.3333362
TAGGTCC604.92307E-448.3333329
Produced by FastQC (version 0.11.5)