FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_Neg_control_Water_SID76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_Neg_control_Water_SID76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATA11.25No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAAGATGAACAA11.25No Hit
CTAATTGATGGCCATCCGAATTATTTTGGTCACTGTCTGGCTGACAGTAA11.25No Hit
CTCTCCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTG11.25No Hit
GATTATTGCTGGTCGCAACATAGTGCGAAGATCTGCAGTATCAGCAGATC11.25No Hit
CATCAGAGGTGTCTCACTGCAGAGCCACAGAATACATAATGAAGGGGGTG11.25No Hit
CTGCTCCAGAACAACGATTAAGTCTCATACTCTTGGCACCACGACACGTT11.25No Hit
GAAGATAAGAGTGCATGAGGGATATGATGAGTTTACAATGGTTGGGAGAA11.25No Hit
TCTTCTTTCAGAATCCGCTCCACTATCTGCTTTCCAGCACGTGTGGCTGT11.25No Hit
AGTTGTGGAGATGCATACAAGCTGACTCGCCTTGCTCATTGATGAAGTGA11.25No Hit
GTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTACATGAAGCATACTTCC11.25No Hit
ATCCATGTGTACGCAAGGAGTTGAACCAATACGCATTAAGCAAAAACCAG11.25No Hit
TTCTTTGCCTGGAGGTTTTCTCAGATGGGAGCACCCCCTTAAGGAAAGCT11.25No Hit
TTTCGTATCAAAATGGGCAAGGCCTTCTTGTACACAACACTCATACTTTG11.25No Hit
CCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTTGACATCCTCAG11.25No Hit
TTAGTGCCTAATGTTTTTAGAGGACAAAACAACGGGAGAGTAAGACAGCT11.25No Hit
AATGCACTGAACGGGGTGTCAAAGACCAGTAGAGGCAACTGTATGGAACA11.25No Hit
CACTGGGATGTTCTTCCAGGTATTTATTTCTCCTTTCGTCAAAAGCAGAG11.25No Hit
GAATGACAAGCATTCAAATGGGACTGTTAAGGACAGAAGCCCTTATAGCT11.25No Hit
ATCTGGGTCTTCAGTTAAAGTGCCAGCATCCTTTCCGAGAACTGTGAGTC11.25No Hit
TCCTTCTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCATCCCCATACT11.25No Hit
AGAGAACTCATCCTCTATGACAACGTAGAAATAAGGCGAATATGGCGCCC11.25No Hit
CTGTAGTGTTGCAAATACTGTTTACTACTGTCCAGGCCATTGAGCGATTT11.25No Hit
ATGCCATTCTGCCGCACTTGAAGATCTAATAGTATTAAGCTTCATTAAAG11.25No Hit
CTCAATTGCTTCATGTAGCCCCCCAAGATCAAACGTCCCAGGATCAAGGT11.25No Hit
AATTAGATCACTCTTTTTGTTCAAACTCTCATTCCGTTTAAATATTGTTA11.25No Hit
ACTCGAATGTGAAGAATCTGTATGAGAAAGTAAAAAGCCAATTAAAGAAT11.25No Hit
CATACCAGCTGTTGTATACCGTCTTTTCTCTTCTTTCACCTCTGTTCTTC11.25No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG11.25No Hit
ATCATACTGAAAGCGAACTTCAGGGTGATTTTTGACCGACTGGAGGATCT11.25No Hit
GATCTTGAACTCTTCTTTCTTTATCTTTCCAGATCCAAAATCAACCCGGC11.25No Hit
ACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAA11.25No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGCAAAAGAT11.25No Hit
AGTTCCCTCTCCATACAACTCGATAATAGAGCCAGACGCTTGGTAAACAA11.25No Hit
GCAGAACACGGATCTTGAGGTTCTCATGGAATGGCTATAGACCTGACACT11.25No Hit
GTTAGCACAATGGCAAGGACAGTCTGGCTAATGGGAGAGAACCCCAAAGC11.25No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT11.25No Hit
GAGCAAAAGCAGGTACTGATCCAAGATGGAAGATTTTGCACAGAAATGCT11.25No Hit
TTATAATACCGCTATGGGATTTCACCGAAGAGAGAGAAATCGCTGGCGAA11.25No Hit
ACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCG11.25No Hit
CTCCAATACTCATGTACACTTACATTTCGCATATAACCACTCCTAAACAA11.25No Hit
TACCAACACGAAGTCCCCTTGACAGAGTAGAAAATAAACAATATAAGCCA11.25No Hit
GTTTCCAGCCGGTCAAAAACTACAATGAAGTTCGCTTTCAGTATGATGAT11.25No Hit
CTGTACTTCTGCGGTGGCAATGCACAGGACAAACCGCCTCGGTTAAGTAA11.25No Hit
AACACCGAGTACTTGAAGATGCACAAATGTACCAAAGGAGCTGCAATTTA11.25No Hit
CTTCTCTCCTCGTTACTCCAATTTCGATGAATCTATTCTCCTTGTAATCA11.25No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11.25No Hit
ATTATCACCCCTCCAGAAGTTCCTATCATCGACACCATGGATGAACATAC11.25No Hit
TTTAGGATCTGCTGCTTGTCCTGTGCCCTCAGAATTTTGATGCCTGAAAC11.25No Hit
CTGTTGAATACCGCTTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGC11.25No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT11.25No Hit
CATTCTGTCTCACATCTTCAGTGATCCATCTTATGTCTTCACAGTTCTGC11.25No Hit
ATACTCCATGCTGGCATCATCACTGCATTGTTCATTGATTCAATTTCTTT11.25No Hit
GTTCATCTTCAAGTACTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGT11.25No Hit
GTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCATACTGGATATC11.25No Hit
ATATAGAGACGAATCGAAAAACACAAGCGTAAACAATATATCATTAAGGA11.25No Hit
GGAACAGGATACACCATGGCACAAATGTACCAAAGGTGCTGCAATTTATT11.25No Hit
CCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAAGTTGTGCTTATAG11.25No Hit
ATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTA11.25No Hit
TATTTACTTATAGGGAGCGCCGAGGGACAGAATGAAATAAAGAGTCTATT11.25No Hit
GATCTTGAACTCTTCTTTCTTTATACTTCCAGATTCGAAATCAACCCGTG11.25No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTAACTAGAAGGATTTTC11.25No Hit
TTTTTCCACCAGCTCTATCCTGACAAAACAACCCAAGACTGCAACATCAG11.25No Hit
GCTTCTTTCTAATGGCAGGCCCTGACTGGAATGGGTCACCGAGGGCCAGA11.25No Hit
AGCTACATGAAGCATACTTCCAAGTGAGTGCTTATTGCTGCAAATTTGTT11.25No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG11.25No Hit
CATCATACTGAAAGCGAACTTCAGTGTGATTTTTGACCGGCTGGAGACTC11.25No Hit
TGGGTGCAGGTAGCCCCTCAGGGACAAAGGGCCAGGGCACAAGGGTGTCC11.25No Hit
AAAACACCTTGAAGCCGGGCCCCCGCGTGTTGGGGGGCTAAACGACGAAA11.25No Hit
GGCTAAAGACACGACCAATCCTGTCACCTCTGACTACAGGGATTTTCGGA11.25No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT11.25No Hit
CAACGAGTAGCTTCTTGTTGCTCTGAAGATTTAACATATAATTGACCTCA11.25No Hit
AAATGCATGAGAACATTCTTTGGATGGAAGGAACCCAATGTTGTTAAACC11.25No Hit
CTGTATTCCCCCTAAGAGCACAAACCGATGTGTGTTGGGGACCGTGCCAC11.25No Hit
CTTTCTCTATGGTGGTGTTATTCAAATACACTATCTCTGTACTGTTCCTT11.25No Hit
CTGTTATGTTCCTTTCTATTATTATTGGTTCACACGGCATTACTTGGTTG11.25No Hit
CACCTAACTGGTATCCAAGCTCTATTCTTCCATATTTATCTCCACGACGG11.25No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTACATTCACTATTTT11.25No Hit
GAACATCCCAGTGCGGGGAAAGATCATAAGAAAACTGGAGGACCTATATA11.25No Hit
GCCCTTCCCTCCTTGATTCCCTCTCTCATTATTAGGATTCCCCCACTTGC11.25No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC50.0145.09
AACTGGT50.0145.06
CCTAACT50.0145.03
CATCAAA50.0145.0145
CTGGTAT50.0145.08
TAACTGG50.0145.05
CACCTAA50.0145.01
CTAACTG50.0145.04
ACCTAAC50.0145.02
ACTGGTA50.0145.07