FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp2_IAV_control_SID75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp2_IAV_control_SID75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145136
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT4490.30936500937052147No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4380.3017859111454085No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG3260.224616911035167No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG3230.22254988424649982No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3220.22186087531694412No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3160.21772682173960978No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA2860.1970565538529379No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2720.18741042883915773No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA2570.17707529489582186No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA2500.17225223238893178No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG2310.15916106272737296No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT2230.15364899129092713No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2200.15158196450225994No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2050.14124683055892404No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2040.14055782162936833No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA2020.13917980377025685No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1880.1295336787564767No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA1850.1274666519678095No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA1780.12264358946091941No Hit
GTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAA1750.12057656267225224No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA1720.11850953588358505No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC1710.11782052695402932No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC1710.11782052695402932No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1610.11093043765847206No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG1590.10955241979936059No Hit
GGGCTAGACTGTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACG1570.10817440194024915No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG1560.10748539301069342No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1530.10541836622202623No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC1530.10541836622202623No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG1510.10404034836291479No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1510.10404034836291479No Hit
AAGTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTT1510.10404034836291479No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA1500.10335133943335906No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT1480.1019733215742476No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGTG100.007084661145.0145
GAGCGAA450.0128.888891
GCAAAAG3400.083.1617663
AGCAAAA3800.074.40792
AGCAGGG3250.073.615398
AAGCAGG4750.071.736847
AGGGTGT1050.069.0476156
GCAGGGT1400.067.321439
GCAGGGG2050.067.195129
CAAAAGC4450.063.539334
AGCGAAA1350.059.074071
CAAGGGT1251.8189894E-1258.04
GTGTTTT1301.8189894E-1255.7692349
CAAGGTA553.2098242E-452.7272729
TTGTGAC752.5397236E-548.3333367
AAGGGTG1509.094947E-1248.3333365
ATATGTA450.00962266548.3333323
TAGGAGA450.00962266548.3333324
AGTAGAG604.9310236E-448.3333322
GAGCAAA6250.047.561
Produced by FastQC (version 0.11.5)