FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID9_Shed_Ind2_D3_rep2_SID29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID9_Shed_Ind2_D3_rep2_SID29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109792
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT10220.9308510638297872No Hit
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG8060.7341154182454095No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT4200.38254153308073446No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC3740.34064412707665404No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT3310.30147916059457885No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG2680.24409793063246868No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2550.23225735937044595No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT2520.2295249198484407No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA2500.22770329350043722No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2220.2022005246283882No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG2180.19855727193238124No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2170.1976464587583795No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2120.19309239288837074No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG2070.18853832701836198No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2030.184895074322355No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG1760.1603031186243078No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1760.1603031186243078No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1750.15939230545030603No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1700.1548382395802973No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA1650.15028417371028854No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1630.14846254736228504No Hit
GTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAGTTTCGG1540.14026522879626932No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1450.13206791023025358No Hit
CCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTG1430.13024628388225007No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTATCCTTCCAGATTCGA1400.12751384436024482No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA1350.12295977849023608No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG1320.12022733896823083No Hit
CCTTTGTTCATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCA1310.11931652579422909No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1250.11385164675021861No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1250.11385164675021861No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1250.11385164675021861No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA1190.1083867677062081No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTT1160.10565432818420285No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1150.10474351501020111No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC1120.10201107548819587No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1100.10018944914019236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA500.0130.51
GCGCAAA203.8567925E-4108.751
AGCAGGC2700.0102.037048
GAGCAAA4050.0100.246911
GCAGGCA2800.098.392869
GCAGGAG301.5930917E-596.6666649
GCAAAAG4650.090.430113
CAAAAGC4950.084.949494
GTAGAAA1550.079.516132
AAGCAGG6250.077.719997
CCCTGCG300.00193113872.5145
TAGTAAA300.00193113872.52
AGTAGAA1850.066.621621
TAGAAAC2000.065.253
AAAGCAG7500.064.766676
CTATGCT350.003558051562.1428579
TGCCCTA350.003558051562.142857145
GTAGTAA606.7654382E-660.4166681
AGCGAAA1102.5465852E-1159.318181
AGCAAAA6950.058.4172632
Produced by FastQC (version 0.11.5)