FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID9_Shed_Ind2_D3_rep1_SID9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID9_Shed_Ind2_D3_rep1_SID9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39824
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT3540.8889112093210125No Hit
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG3460.8688228204098031No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1630.4093009240658899No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1330.33396946564885494No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG1020.25612695861791884No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1000.2511048613901165No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT980.24608276416231417No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA890.22348332663720372No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA860.21595018079550019No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA840.21092808356769788No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA820.20590598633989554No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT810.2033949377259944No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG750.1883286460425874No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT740.18581759742868623No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA710.17828445158698272No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA710.17828445158698272No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG670.16824025713137808No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG580.14564081960626757No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA570.1431297709923664No Hit
CCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTG560.14061872237846523No Hit
GTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAGTTTCGG550.13810767376456407No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA500.12555243069505825No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG470.11801928485335475No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTATCCTTCCAGATTCGA460.1155082362394536No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT460.1155082362394536No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG450.11299718762555243No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT440.11048613901165126No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC440.11048613901165126No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTT430.10797509039775009No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC430.10797509039775009No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT430.10797509039775009No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA420.10546404178384894No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT420.10546404178384894No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT410.10295299316994777No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC400.1004419445560466No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACAT100.007065483145.000023
GTAATCC100.007065483145.000022
GAGCGAA600.0145.01
AGCAGGC750.0125.666678
GCAGGCA800.0117.812519
AAGCAGG2300.0100.869577
GAGCAAA1450.0100.01
AGCGAAA900.096.6666642
GCAAAAG1600.095.156263
CGAAAGC900.088.6111154
CTCACCG259.3201856E-487.03
CGTGCCC259.3201856E-487.08
AGCAGGG608.021743E-1084.5833368
AAAGCAG2800.082.857146
CAAAAGC1850.082.29734
TCACCGT300.001922066672.54
CCGTGCC300.001922066672.57
GCAGGGT502.2657514E-672.59
TTACCCG300.001922066672.5145
AGCAGGT1200.072.58
Produced by FastQC (version 0.11.5)