FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID7_Shed_Ind3_D2_rep1_SID7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID7_Shed_Ind3_D2_rep1_SID7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116061
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT6500.5600503183670655No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT4910.4230533943357372No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC3880.3343069592714176No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2730.23522113371416756No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2580.22229689559800447No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2460.21195750510507408No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2410.2076494257330197No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2410.2076494257330197No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT1660.14302823515220445No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1480.12751914941280879No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG1470.1266575335383979No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1410.12148783829193269No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1360.11717975891987834No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1360.11717975891987834No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1330.11459491129664572No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1270.10942521605018052No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1260.10856360017576965No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1250.10770198430135877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA501.8189894E-12116.01
GTAGTAT259.365703E-487.06
GAGCAAA3800.083.947371
AGCAGGC2050.081.341468
CAAAAGC4550.071.703294
GCAAAAG4700.069.4148943
AAGCAGG5850.065.683767
GCAGGCA2600.064.134619
CGGCAGG502.0028149E-458.06
ACGGCAG502.0028149E-458.05
CCATAGG502.0028149E-458.04
CGAAAGC1153.8198777E-1156.7391324
TAGGTAC400.0060374754.3757
TATCTTG400.0060374754.375145
CTACGGC553.2071397E-452.7272763
GGCTACG553.2071397E-452.7272761
AGCGAAA1258.731149E-1152.2000051
ACAAGGG701.6866321E-551.7857133
GCAGGGT1301.2914825E-1050.1923079
AAAGCAG7850.049.8726166
Produced by FastQC (version 0.11.5)