FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID6_Shed_Ind2_D2_rep2_SID26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID6_Shed_Ind2_D2_rep2_SID26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154197
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG11230.7282891366239291No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT7190.4662866333326848No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA5330.34566171845107235No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT5080.3294486922573072No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4880.3164782713022951No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4640.3009137661562806No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA4600.2983196819652782No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4510.29248299253552273No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3680.23865574557222258No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTTCATCTTCAA3600.2334675771902177No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3480.22568532461721044No Hit
GAAGACAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGA3320.2153089878532008No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3240.21012081947119596No Hit
CTTTTCTTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACC3160.2049326510891911No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT3080.19974448270718625No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG3000.19455631432518142No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2980.1932592722296802No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2890.18742258279992477No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2830.18353145651342112No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA2710.17574920394041388No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG2630.17056103555840907No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2590.16796695136740664No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2230.14462019364838485No Hit
GACAAGTCAAAGAGGAGTACTTGAAGATGAACAAAGGTGCTGCAATTTAT2230.14462019364838485No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2210.14332315155288364No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2170.14072906736188123No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2090.1355408989798764No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2070.13424385688437518No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2060.13359533583662458No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2040.13229829374112337No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2030.13164977269337277No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT2000.12970420955012096No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1960.12711012535911853No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1960.12711012535911853No Hit
GTATTCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC1950.1264616043113679No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1920.12451604116811611No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1860.12062491488161248No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1750.11349118335635584No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA1730.11219414126085463No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT1650.10700597287884978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA700.0124.285721
ATTGCAC352.7505848E-7103.571434145
GAGCAAA6350.086.771661
AGCAGGC1450.085.08
TTAGTAG353.4280412E-582.857151
GCAAAAG6700.082.238813
AAGCAGG8150.077.392647
CAAAAGC7150.077.0629354
GCAGGAG502.2969853E-672.59
TCTTGGA1250.069.65
GCAGGGT1800.068.472239
GCAGGTC1700.068.23539
TAAACTA1009.094947E-1265.255
CAAGGTC451.1906692E-464.444458
AGCGAAA1500.062.8333321
AGCAGGG2900.062.58
CTGTCCT350.00356078462.14286145
AGCAGGT4700.061.702138
TATACTC502.0048904E-458.05
GTAGAAA2250.054.777782
Produced by FastQC (version 0.11.5)