FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID6_Shed_Ind2_D2_rep1_SID6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID6_Shed_Ind2_D2_rep1_SID6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173691
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG9560.5504027266812903No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT6010.34601677691993254No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA4870.2803829789683979No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4470.2573535761783857No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT4460.25677784110863544No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA4220.24296019943462815No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3840.22108226678411663No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3780.2176278563656148No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3110.17905360669234446No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG2950.16984184557633958No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTTCATCTTCAA2790.16063008446033472No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT2770.15947861432083413No Hit
GAAGACAGCAGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGA2700.155448468832582No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG2680.1542969986930814No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2600.14969111813507896No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2580.14853964799557837No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2540.14623670771657715No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA2540.14623670771657715No Hit
CTTTTCTTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACC2490.1433580323678256No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2310.13299480111232015No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2230.12838892055431772No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG2160.1243587750660656No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2110.12148009971731408No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2080.11975289450806316No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2070.11917715943831288No Hit
GACAAGTCAAAGAGGAGTACTTGAAGATGAACAAAGGTGCTGCAATTTAT1920.11054113339205832No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1910.109965398322308No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1880.1082381931130571No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1840.10593525283405587No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1790.10305657748530438No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1770.10190510734580376No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA100.0070858533145.0145
AGCAGGC1250.098.68
GAGCGAA900.088.6111151
GTATAGT259.373598E-487.04
GAGCAAA5650.078.274341
GCAAAAG6300.075.9523853
AAGCAGG7400.075.439197
CAAAAGC6350.075.3543244
AGCAGGG2500.072.58
GTATATA808.034476E-963.43751
GCAGGGT2100.062.1428579
AATGTAT350.00356154462.1428571
GCAGGCA2150.057.325589
CGAAAGC1900.057.2368433
ATGTATA1400.056.9642872
CAAGGTA1153.8198777E-1156.7391328
GGATAGC1450.055.05
AGCGAAA1850.054.864871
TTATATA400.006042502454.3754
TTAGTAG400.006042502454.3751
Produced by FastQC (version 0.11.5)