FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID5_Shed_Ind1_D2_rep1_SID5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID5_Shed_Ind1_D2_rep1_SID5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116828
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3300.28246653199575444No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2580.22083747046940805No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1850.15835244975519566No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1740.14893689868867052No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1730.14808093950080461No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1530.13096175574348615No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1520.1301057965556202No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1520.1301057965556202No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1490.12753791899202246No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1420.12154620467696099No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1400.11983428630122916No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1370.1172664087376314No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1210.10357106173177663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGAG352.746383E-7103.571439
GAGCGAA608.203642E-1084.5833361
GAGCAAA2950.071.271191
GCAGGGT1100.065.9090969
AAGCAGG4200.065.5952457
TATGCTA451.18946526E-464.444442
GCTAGAG451.18946526E-464.444445
AGCAGGG1700.059.705888
GCAAAAG3800.057.2368433
CAAAAGC3950.055.0632934
CACTGTA400.006037567354.3757
TATAGGT701.6867E-551.7857134
TAGGTCC858.85655E-751.1764686
AGCGAAA1056.836308E-848.3333362
CTAGAGT604.9270445E-448.3333326
CGAAAGC901.3134722E-648.3333323
CTATATA604.9270445E-448.3333322
GCACTGT450.00961799848.3333326
GACAAGT657.309898E-444.6153877
AGGGTGT1002.7133556E-643.56
Produced by FastQC (version 0.11.5)