FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID4_Shed_Ind3_D1_rep2_SID24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID4_Shed_Ind3_D1_rep2_SID24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60052
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1070.17817891160993804No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA830.13821354825817625No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC690.1149004196363152No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT630.10490907879837474No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT610.1015786318523946No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT610.1015786318523946No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT610.1015786318523946No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT610.1015786318523946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT100.0070743924145.06
TCTAGAG100.0070743924145.02
GAGCGAA203.8481917E-4108.751
GGGTAAC301.5871867E-596.6666647
GCAGGAG259.34353E-487.07
TAAGGGT451.18477685E-464.444444
AAGCAGG2550.059.7058877
GCAGGGT1102.3646862E-1159.318189
GTAACAG501.9949971E-458.09
AGCAGGT1153.8198777E-1156.739138
GCAAAAG2200.056.0227243
CAAAAGC2250.054.777784
GCAGGTC400.006023335754.3759
AGCAGGG1600.054.3758
GAGCAAA2150.053.9534871
GGTAACA553.194658E-452.7272728
TTGCTAA553.194658E-452.7272729
CAGTTCG553.194658E-452.7272729
AGGGTAA553.194658E-452.7272726
AAGGGTA553.194658E-452.7272725
Produced by FastQC (version 0.11.5)