FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID4_Shed_Ind3_D1_rep1_SID4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID4_Shed_Ind3_D1_rep1_SID4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83440
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1890.22651006711409397No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1530.18336529242569513No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1210.1450143815915628No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1140.13662511984659634No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1100.1318312559923298No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA930.11145733461169703No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAT100.0070792963145.0145
AAGGGTG1000.072.55
AGGGTGT1100.065.909096
GAGCAAA2350.064.787231
GAGCGAA451.1874768E-464.444451
AGAGTCT702.3084613E-762.1428576
CAAGGGT1200.060.4166684
CAAAAGC2800.059.553574
GCAAAAG2800.059.553573
AAGCAGG3200.056.6406257
TATATAG651.08492895E-555.769235
CTAGTAG651.08492895E-555.769233
GCAGGAG400.00603153454.3759
ATAGTGT400.00603153454.3759
TAGGAAG805.8083606E-754.3754
AGCAGGC400.00603153454.3758
GGTGTTT1353.6379788E-1253.7037058
GGGTGTT1405.456968E-1251.7857137
AGCAGGG1405.456968E-1251.7857138
ACAAGGG1405.456968E-1251.7857133